Blasticidin S HCl
(Synonyms: 灭瘟素S盐酸盐) 目录号 : GC18094Blasticidin S HCl是一种从灰色链霉菌中分离的核苷类抗生素。
Cas No.:3513-03-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment [1]: |
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Cell lines |
HeLa cells |
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Preparation method |
Cells were seeded in six-well-plates and transfected after 24 h with 0.6 μg/well of the reporter plasmids pCI-Renilla-HBB WT or pCI-Renilla-HBB-Nonsense-Mutation 39 (NS39).After 16 h, the cells were washed with full medium and treated for 3 h with Blasticidin S HCl or untreated. |
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Reaction Conditions |
0-100μg/ml; 3 h |
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Applications |
Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells. |
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References: [1]. Powers KT, Stevenson-Jones F,et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960. |
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Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1]. Blasticidin S HCl specifically inhibits protein synthesis in prokaryotic or eukaryotic cells by interfering with ribosome binding peptides. The inhibitory effect of Blasticidin S HCl on translation termination is conserved in eukaryotes and bacteria[2-4].
Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells, Blasticidin S HCl inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production[5]. The presence of Blasticidin S HCl protects DNA from Cu(II) -induced damage[6].
References:
[1]. TAKEUCHI S, HIRAYAMA K, et,al. Blasticidin S, a new antibiotic. J Antibiot (Tokyo). 1958 Jan;11(1):1-5. PMID: 13525246.
[2]. Svidritskiy E, Korostelev AA. Mechanism of Inhibition of Translation Termination by Blasticidin S. J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. PMID: 29366636; PMCID: PMC5831496.
[3]. Kinoshita T, Tanaka N, et,al. Binding of blasticidin S to ribosomes. J Antibiot (Tokyo). 1970 Jun;23(6):288-90. doi: 10.7164/antibiotics.23.288. PMID: 4917792.
[4]. Svidritskiy E, Ling C, et,al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12283-8. doi: 10.1073/pnas.1304922110. Epub 2013 Jul 3. PMID: 23824292; PMCID: PMC3725078.
[5]. Powers KT, Stevenson-Jones F, et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960.
[6]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem. 2013 Oct;127:73-8. doi: 10.1016/j.jinorgbio.2013.06.014. Epub 2013 Jun 29. PMID: 23880034.
Blasticidin S HCl是一种从灰色链霉菌中分离的核苷类抗生素[1]。Blasticidin S HCl通过干扰核糖体结合肽段而特异性抑制原核细胞或真核细胞的蛋白合成。Blasticidin S HCl对翻译终止的抑制作用在真核生物和细菌中是保守的[2-4]。
Blasticidin S HCl处理能够稳定HeLa细胞中过早终止密码子突变的mRNA, Blasticidin S HCl抑制无意义介导的mRNA衰变，并且在亚抑制浓度下，调节过早终止密码子的翻译动力学，从而增强蛋白质产生[5]。Blasticidin S HCl的存在可以保护DNA免受Cu(II)诱导的损伤[6]。
| Cas No. | 3513-03-9 | SDF | |
| 别名 | 灭瘟素S盐酸盐 | ||
| 化学名 | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride | ||
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl | ||
| 分子式 | C17H27ClN8O5 | 分子量 | 458.9 |
| 溶解度 | Water: 92 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1791 mL | 10.8956 mL | 21.7912 mL |
| 5 mM | 0.4358 mL | 2.1791 mL | 4.3582 mL |
| 10 mM | 0.2179 mL | 1.0896 mL | 2.1791 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。