Bleomycin A5 (hydrochloride)
(Synonyms: 盐酸平阳霉素,Pingyangmycin) 目录号 : GC10909
Bleomycin A5 (Pingyangmycin) hydrochloride 是一种抗肿瘤糖蛋白抗生素。
Cas No.:55658-47-4
Sample solution is provided at 25 µL, 10mM.
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Bleomycin A5 is an anticancer chemotherapeutic that binds DNA and induces DNA cleavage and strand breaks, it is highly toxic. Additionally, this compound induces apoptosis, alters cell cycle regulation, inhibits proliferation, and decreases tumor size in cellular and animal models of hemangioma. Bleomycin may also inhibit thioredoxin reductase.
In vitro: The IC50 of BLM alone to the cells was about 2.63 μg/ml. The IC50 of BLM was decreased to 1/3.8 and 1/9.5 of 2.63 μg/ml when used in combination with 2.63 μg/ml and 5 μg/ml W-131 [1]. Bleomycin A5 induced the activation of a JNK pathway in a dose-dependent manner. After 24h treatment, Bleomycin A5 induced dramatic cell death. DAPI nuclear staining assay revealed that PD98059 (10 μM) markedly enhanced bleomycin A5-induced apoptosis [2].
In vivo: In the rats, Bleomycin A5 induced pulmonary inflammation and fibrosis, increased the levels of malondialdehyde and tumour necrosis factor-α and enhanced accumulation of collagen in the lungs. Rolipram administration significantly attenuated these effects [3].
Clinical Trials: Bleomycin is clinically used to treat lymphomas, squamous cell carcinomas, and testicular cancer.
References:
[1]. Zhang H Q, He N G, Xue S B. Effect of bleomycin A5 with calmodulin inhibitor on the proliferation of S-180 cells in vitro[J]. Zhongguo yao li xue bao= Acta pharmacologica Sinica, 1990, 11(5): 470-473.
[2]. Yang L C, Yang S H, Tai K W, et al. MEK inhibition enhances bleomycin A5‐induced apoptosis in an oral cancer cell line: signaling mechanisms and therapeutic opportunities[J]. Journal of oral pathology & medicine, 2004, 33(1): 37-45.
[3]. Pan J B, Hou Y H, Zhang G J. Rolipram attenuates bleomycin A5‐induced pulmonary fibrosis in rats[J]. Respirology, 2009, 14(7): 975-982.
| Cas No. | 55658-47-4 | SDF | |
| 别名 | 盐酸平阳霉素,Pingyangmycin | ||
| 化学名 | N1-[3-[(4-aminobutyl)amino]propyl]-bleomycinamide, hydrochloride | ||
| Canonical SMILES | CC(C(N1)=N)=C(N=C1[C@](NC[C@@](N)([H])C(O)=N)([H])CC(O)=N)/C(O)=N/[C@@](/C(O)=N/[C@]([C@](O)([H])[C@@](/C(O)=N/[C@@](/C(O)=N/CCC2=NC(C3=NC(/C(O)=N/CCCNCCCCN)=CS3)=CS2)([H])[C@@](O)([H])C)([H])C)([H])C)([H])[C@](O[C@@]4([H])[C@](O[C@]5([H])[C@](O)([H])[C@] | ||
| 分子式 | C57H89N19O21S2 • XHCl | 分子量 | 1477 |
| 溶解度 | DMSO: 0.1 mg/mL,PBS (pH 7.2): 10 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.677 mL | 3.3852 mL | 6.7705 mL |
| 5 mM | 0.1354 mL | 0.677 mL | 1.3541 mL |
| 10 mM | 0.0677 mL | 0.3385 mL | 0.677 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >85.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet