BODIPY 493/503
(Synonyms: 4,4-二氟-1,3,5,7,8-五甲基-4-硼-3A,4A-二氮杂-S-茚烯,Pyrromethene 546; BDP 493/503 lipid stain) 目录号 : GC42959
BODIPY 493/503是一种亲脂性荧光染料，发出明亮的绿色荧光，被广泛用于标记脂质滴（激发/发射：493/503纳米）。
Cas No.:121207-31-6
Sample solution is provided at 25 µL, 10mM.
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BODIPY 493/503, a lipophilic fluorescence dye, emits bright green fluorescence has been used extensively for lipid droplet labeling (Ex/Em: 493/503 nm) [1,2]. BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry, and can be used for live and fixed cell applications.
References:
[1]. Ohsaki Y, Shinohara Y, Suzuki M, et al. A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy[J]. Histochemistry and cell biology, 2010, 133(4): 477-480.
[2]. Listenberger, L.L., Studer, A.M., Brown, D.A., and Wolins, N.E. 2016. Fluorescent detection of lipid droplets and associated proteins. Curr. Protoc. Cell Biol. 71:4.31.1-4.31.14. doi: 10.1002/cpcb.7
BODIPY 493/503是一种亲脂性荧光染料，发出明亮的绿色荧光，被广泛用于标记脂质滴（激发/发射：493/503纳米）[1,2]。BODIPY 493/503适用于表面荧光显微镜、共聚焦显微镜、双光子显微镜以及流式细胞术，并可用于活体和固定细胞应用。
本方案仅提供一个指导，请根据您的具体需要进行修改。
1.染色液的制备
(1)配置储存液:使用DMSO溶解BODIPY 493/503，配置浓度为1-5mM的储存液。
注意:未使用的储存液分装后在-20℃或-80°C避光保存，避免反复冻融。
(2)配置工作液:用合适的缓冲液(如:无血清培养基或PBS)稀释储存液，配制浓度为1-5μM的工作液。
注意:请根据实际情况调整工作液浓度，现用现配。
2.细胞悬浮染色
(1)悬浮细胞：经4°C、1000g离心3-5分钟，弃去上清液，用PBS清洗两次，每次5分钟。
(2)贴壁细胞：使用PBS清洗两次，加入胰酶消化细胞，消化完成后经1000g离心3-5min。
(3)加入1mL的BODIPY 493/503工作溶液重悬细胞，37 °C避光孵育5-30分钟。不同细胞最佳孵育时间不同，请根据具体实验需求自行摸索。
(4)孵育结束后，经1000g离心5分钟，去除上清液，加入PBS清洗2-3次，每次5分钟。
(5)用预温的无血清细胞培养基或PBS重悬细胞，通过荧光显微镜或流式细胞术观察。
3.细胞贴壁染色
(1)在无菌盖玻片上培养贴壁细胞。
(2)从培养基中移走盖玻片，吸出过量的培养基，将盖玻片放在潮湿的环境中。
(3)从盖玻片的一角加入100μL的染料工作液，轻轻晃动使染料均匀覆盖所有细胞。
(4) 37 °C避光孵育5-30分钟。不同细胞最佳孵育时间不同，请根据具体实验需求自行摸索。
(5)孵育结束后吸弃染料工作液，使用预温的培养液清洗盖玻片2~3次。
4.显微镜检测：BODIPY 493/503的最大激发/发射光分别为493/503 nm。
注意事项：
①建议设置阳性对照，对照组细胞使用30μM油酸孵育8小时后进行后续实验；
②荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭；
③为了您的安全和健康，请穿实验服并戴一次性手套操作。
| Cas No. | 121207-31-6 | SDF | |
| 别名 | 4,4-二氟-1,3,5,7,8-五甲基-4-硼-3A,4A-二氮杂-S-茚烯,Pyrromethene 546; BDP 493/503 lipid stain | ||
| 化学名 | (T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)ethyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron | ||
| Canonical SMILES | [F-][B+3]([N-]1C2=C(C)C=C1C)([N](C(C(C)=C3)=C2C)=C3C)[F-] | ||
| 分子式 | C14H17BF2N2 | 分子量 | 262.1 |
| 溶解度 | DMSO : 5 mg/mL (19.08 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.8153 mL | 19.0767 mL | 38.1534 mL |
| 5 mM | 0.7631 mL | 3.8153 mL | 7.6307 mL |
| 10 mM | 0.3815 mL | 1.9077 mL | 3.8153 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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