Bradykinin Fragment (1-5) (trifluoroacetate salt)
(Synonyms: Arg-Pro-Pro-Gly-Phe, BK1-5, RPPGF) 目录号 : GC52424A metabolite of bradykinin
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Bradykinin fragment (1-5) is a metabolite of the endogenous vasodilator bradykinin .1 It is formed from bradykinin by angiotensin-converting enzyme (ACE). Bradykinin fragment (1-5) (100 nM) increases the production of nitric oxide (NO) in neonatal rat cardiomyocytes.2 It inhibits α-thrombin-induced platelet aggregation of washed isolated platelets (IC50 = 0.5 mM).3 Bradykinin fragment (1-5) prevents decreases in mean arterial blood pressure and heart rate and increases survival in a rat model of LPS-induced septicemia.4
1.Bujak-Gi?ycka, B., Olszanecki, R., Madej, J., et al.Metabolism of bradykinin in aorta of hypertensive ratsActa Biochim. Pol.58(2)199-202(2011) 2.Souza-Silva, I.M., de Paula, C.A., Bolais-Amos, L., et al.Peptide fragments of bradykinin show unexpected biological activity not mediated by B1 or B2 receptorsBr. J. Pharmacol.179(12)3061-3077(2022) 3.Hasan, A.A.K., Amenta, S., and Schmaier, A.H.Bradykinin and its metabolite, Arg-Pro-Pro-Gly-Phe, are selective inhibitors of α-thrombin-induced platelet activationCirculation94(3)517-528(1996) 4.Morinelli, T.A., Webb, J.G., Jaffa, A.A., et al.A metabolic fragment of bradykinin, Arg-Pro-Pro-Gly-Phe, protects against the deleterious effects of lipopolysaccharide in ratsJ. Pharmacol. Exp. Ther.296(1)71-76(2001)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7461 mL | 8.7306 mL | 17.4611 mL |
| 5 mM | 0.3492 mL | 1.7461 mL | 3.4922 mL |
| 10 mM | 0.1746 mL | 0.8731 mL | 1.7461 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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