Brassinolide
(Synonyms: 油菜素内酯; Brassin lactone) 目录号 : GC11632
A plant growth regulator
Cas No.:72962-43-7
Sample solution is provided at 25 µL, 10mM.
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IC50: N/A
Brassinolide is a plant growth regulator. Plant growth regulators are naturally produced by plants and are critical for regulating their own growth. Plant growth regulators act via modifying or controlling plant growth processes, such as leave and flower formation, stem elongation, as well as fruit development and ripening.
In vitro: Brassinolide induced the time and concentration-dependent cytotoxicity in PC-3 cells. The mode of such cell death appeared to be apoptosis predominately, as demonstrated by fluorescence, flow-cytometric analyses and transmission electron microscopes. Moreover, Caspase-3 activity was increased after brassinolide treatment obviously. Western blot studies showed brassinolide treatment triggered a time-dependent decrease in the expression of Bcl-2 [1].
In vivo: Brassinolide had the similar function of reducing the blood glucose levels as phenformin, but without the dose-dependent manner. The blood glucose levels showed significant differences after brassinolide treatment with different doses (200, 100, and 50 mg/kg). These results indicated that brassinolide could reduce the blood glucose levels without toxicity [2].
Clinical trial: So far, no clinical study has been reported.
References:
[1] Wu YD,Lou YJ. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie.2007 May;62(5):392-5.
[2] CHEN Shi-ping, HE Jia, WANG Qi-jing, WANG Jian-dong, YE Yan-li, XIAN Li-jian. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats. Acta Metallurgica Sinica, 2009,V26(03): 21-23
| Cell experiment [1]: | |
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Cell lines |
Human prostate cancer PC-3 cell |
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Preparation method |
The solubility of this compound in DMSO is >24.1mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
10, 20 and 40 μM; 12, 24 and 36 h |
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Applications |
Brassinolide induced a time and concentration-dependent cytotoxicity in PC-3 cells. Brassinolide (10, 20 and 40 μM, 12 h) induced a concentration-dependent increase in the apoptotic rate and marked accumulation in G2/M phase of cell cycle. PC-3 cells treated with brassinolide (20 μM, 24 h) showed characteristic apoptotic alterations: shrinking cellular figure, decreasing cell surface microvilli, cytoplasmic vacuoles, chromatin condensation. PC-3 cells treated with brassinolide (20 μM) for 6, 12 and 18 h showed a time-dependent increase in the activity of caspases-3. |
| Animal experiment [2]: | |
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Animal models |
Diabetes rats |
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Dosage form |
Oral administration; 200,100,and 50 mg/kg; once every day for 7 days |
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Application |
Oral administration of Brassinolide decreased the levels of blood glucose from 19.71-24.10 mmol/L to 9.89 mmol/L-12.70 mmol/L. The levels of blood glucose displayed significant differences after treatment with different dose of brassinolide. Brassinolide can still reduce the blood glucose levels without toxicity effect even at a lower dose. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Wu Y D, Lou Y J. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells[J]. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 2007, 62(5): 392-395. [2]. CHEN S, HE J, WANG Q, et al. Effect of Brassinolide on Levels of Blood Glucose in Alloxan—induced Diabetes Rats [J][J]. Laboratory Animal Science, 2009, 3: 007. |
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| Cas No. | 72962-43-7 | SDF | |
| 别名 | 油菜素内酯; Brassin lactone | ||
| 化学名 | (3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-((2S,3R,4R,5S)-3,4-dihydroxy-5,6-dimethylheptan-2-yl)-5,6-dihydroxy-7a,9a-dimethyltetradecahydro-1H-benzo[c]indeno[5,4-e]oxepin-3(12bH)-one | ||
| Canonical SMILES | CC(C)[C@H](C)[C@@H](O)[C@H](O)[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])COC([C@@]4([H])C[C@H](O)[C@H](O)C[C@]4(C)[C@@]3([H])CC[C@@]21C)=O | ||
| 分子式 | C28H48O6 | 分子量 | 480.68 |
| 溶解度 | ≥ 48.1 mg/mL in DMSO with gentle warming, ≥ 52.3 mg/mL in EtOH with ultrasonic and warming | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0804 mL | 10.4019 mL | 20.8039 mL |
| 5 mM | 0.4161 mL | 2.0804 mL | 4.1608 mL |
| 10 mM | 0.208 mL | 1.0402 mL | 2.0804 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet