Brentuximab vedotin
(Synonyms: 维布妥昔单抗；本妥昔单抗) 目录号 : GC68296Brentuximab vedotin (cAC10-vcMMAE) 是一种抗体-药物偶联物 (ADC)，由抗 CD30 抗体和 Monomethyl auristatin E (MMAE) 组成。Brentuximab vedotin 抑制 CD30 阳性细胞，其 IC50 值为 2.5 ng/mL。Brentuximab vedotin 可用于复发难治性霍奇金淋巴瘤的研究。
Cas No.:914088-09-8
Sample solution is provided at 25 µL, 10mM.
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IC50: 2.5 ng/mL (CD30)[2]
Brentuximab vedotin (cAC10-vcMMAE) is an antibody-drug conjugate (ADC) comprising an anti-CD30 antibody and the cytotoxic agent Monomethyl auristatin E (MMAE). Brentuximab vedotin inhibits CD30-positive cells with an IC50 of 2.5 ng/mL. Brentuximab vedotin can be used for the research of relapsed and refractory Hodgkin lymphoma[1][2].
Brentuximab vedotin (cAC10-vcMMAE) (1 μg/mL; 96 h) shows cytotoxicity to CD30+ in Karpas 299 cells[2].
Brentuximab vedotin (CAC10-VCMMAE) (1 μg/mL; 12, 24 and 48 h) selectively induces growth arrest in G2/M phase then lead to apoptotic cell death[2].
Cell Cytotoxicity Assay[2]
| Cell Line: | Karpas 299 cells |
| Concentration: | 1 μg/mL |
| Incubation Time: | 96 h |
| Result: | Showed cytotoxicity to CD30+ Karpas 299 cells with an IC50 value of 2.5 ng/mL. |
Cell Cycle Analysis[2]
| Cell Line: | L540 cells |
| Concentration: | 1 μg/mL |
| Incubation Time: | 12, 24, and 48 h |
| Result: | Selectively induced growth arrest in G2/M phase to apoptotic cell death. |
Brentuximab vedotin (cAC10-vcMMAE) (10-120 mg/kg; i.p. for 3 weeks) the maximum tolerated dose (MTD) is between 30 and 40 mg/kg[2].
Brentuximab vedotin (cAC10-vcMMAE) (0.3, 1 mg/kg; flanks injection; every 4 days for a total of 4 doses 1 mg/kg) induces tumor CD30 regression[2].
| Animal Model: | SCID mice[2] |
| Dosage: | 10 to 120 mg/kg |
| Administration: | Intravenous injection; 10 to 120 mg/kg; for 3 weeks |
| Result: | Showed an maximum tolerated dose between 30 and 40 mg/kg. |
| Animal Model: | SCID mice[2] |
| Dosage: | 0.3 and 1 mg/kg |
| Administration: | Flanks injection; 1 mg/kg every 4 days for a total of 4 doses; 0.3 mg/kg every 4 days for a total of 4 doses |
| Result: | Induced complete and durable tumor regression, but 0.3 mg/kg provided lower therapy than 1 mg/kg dose. |
[1]. Shea L, Mehta-Shah N. Brentuximab Vedotin in the Treatment of Peripheral T Cell Lymphoma and Cutaneous T Cell Lymphoma. Curr Hematol Malig Rep. 2020 Feb;15(1):9-19.
[2]. Francisco JA, et al. cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity. Blood. 2003 Aug 15;102(4):1458-65.
| Cas No. | 914088-09-8 | SDF | Download SDF |
| 别名 | 维布妥昔单抗；本妥昔单抗 | ||
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | -80°C,protect from light | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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