Bromelain
(Synonyms: 菠萝蛋白酶) 目录号 : GC62536
A mixture of proteolytic enzymes
Cas No.:9001-00-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Bromelain is a mixture of proteolytic enzymes and non-protease components (phosphatases, glucosidases, peroxidases, cellulases, glycoproteins, and carbohydrates) derived from the stem and fruit of pineapple plants.1,2 Bromelain has been studied for a number of potentially beneficial effects, including anti-inflammatory, anti-thrombotic, fibrinolytic, and anti-cancer functions.1,2
1.Chobotova, K., Vernallis, A.B., and Majid, F.A.A.Bromelain's activity and potential as an anti-cancer agent: Current evidence and perspectivesCancer Lett.290(2)148-156(2010) 2.Yuan, G., Wahlqvist, M.L., He, G., et al.Natural products and anti-inflammatory activityAsia Pac.J.Clin.Nutr.15(2)143-152(2006)
| Cas No. | 9001-00-7 | SDF | |
| 别名 | 菠萝蛋白酶 | ||
| 分子式 | 分子量 | ||
| 溶解度 | DMSO : 6.67 mg/mL (ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Beneficial Properties of Bromelain
Nutrients 2021 Nov 29;13(12):4313.PMID:34959865DOI:10.3390/nu13124313.
Bromelain is a major sulfhydryl proteolytic enzyme found in pineapple plants, having multiple activities in many areas of medicine. Due to its low toxicity, high efficiency, high availability, and relative simplicity of acquisition, it is the object of inexhaustible interest of scientists. This review summarizes scientific reports concerning the possible application of Bromelain in treating cardiovascular diseases, blood coagulation and fibrinolysis disorders, infectious diseases, inflammation-associated diseases, and many types of cancer. However, for the proper application of such multi-action activities of Bromelain, further exploration of the mechanism of its action is needed. It is supposed that the anti-viral, anti-inflammatory, cardioprotective and anti-coagulatory activity of Bromelain may become a complementary therapy for COVID-19 and post-COVID-19 patients. During the irrepressible spread of novel variants of the SARS-CoV-2 virus, such beneficial properties of this biomolecule might help prevent escalation and the progression of the COVID-19 disease.
Stability, purification, and applications of Bromelain: A review
Biotechnol Prog 2016 Jan-Feb;32(1):5-13.PMID:26518672DOI:10.1002/btpr.2190.
Bromelain is a cysteine protease found in pineapple tissue. Because of its anti-inflammatory and anti-cancer activities, as well as its ability to induce apoptotic cell death, Bromelain has proved useful in several therapeutic areas. The market for this protease is growing, and several studies exploring various properties of this molecule have been reported. This review aims to compile this data, and summarize the main findings on Bromelain in the literature to date. The physicochemical properties and stability of Bromelain under different conditions are discussed. Several studies on the purification of Bromelain from crude extracts using a wide range of techniques such as liquid-liquid extractions by aqueous two-phase system, ultrafiltration, precipitation, and chromatography, have been reported. Finally, the various applications of Bromelain are presented. This review therefore covers the main properties of Bromelain, aiming to provide an up-to-date compilation of the data reported on this enzyme.
Bromelain: biochemistry, pharmacology and medical use
Cell Mol Life Sci 2001 Aug;58(9):1234-45.PMID:11577981DOI:10.1007/PL00000936.
Bromelain is a crude extract from the pineapple that contains, among other components, various closely related proteinases, demonstrating, in vitro and in vivo, antiedematous, antiinflammatory, antithrombotic and fibrinolytic activities. The active factors involved are biochemically characterized only in part. Due to its efficacy after oral administration, its safety and lack of undesired side effects, Bromelain has earned growing acceptance and compliance among patients as a phytotherapeutical drug. A wide range of therapeutic benefits has been claimed for Bromelain, such as reversible inhibition of platelet aggregation, angina pectoris, bronchitis, sinusitis, surgical traumas, thrombophlebitis, pyelonephritis and enhanced absorption of drugs, particularly of antibiotics. Biochemical experiments indicate that these pharmacological properties depend on the proteolytic activity only partly, suggesting the presence of nonprotein factors in Bromelain. Recent results from preclinical and pharmacological studies recommend Bromelain as an orally given drug for complementary tumor therapy: Bromelain acts as an immunomodulator by raising the impaired immunocytotoxicity of monocytes against tumor cells from patients and by inducing the production of distinct cytokines such as tumor necrosis factor-a, interleukin (Il)-1beta, Il-6, and Il-8. In a recent clinical study with mammary tumor patients, these findings could be partially confirmed. Especially promising are reports on animal experiments claiming an antimetastatic efficacy and inhibition of metastasis-associated platelet aggregation as well as inhibition of growth and invasiveness of tumor cells. Apparently, the antiinvasive activity does not depend on the proteolytic activity. This is also true for Bromelain effects on the modulation of immune functions, its potential to eliminate burn debris and to accelerate wound healing. Whether Bromelain will gain wide acceptance as a drug that inhibits platelet aggregation, is antimetastatic and facilitates skin debridement, among other indications, will be determined by further clinical trials. The claim that Bromelain cannot be effective after oral administration is definitely refuted at this time.
Bromelain
Plast Reconstr Surg 2006 Dec;118(7):1640-1644.PMID:17102739DOI:10.1097/01.prs.0000242503.50548.ee.
Bromelain may be of interest to plastic surgeons because of its apparent ability to reduce pain, edema, inflammation, and platelet aggregation, as well as its ability to potentiate antibiotics, which may be beneficial in postoperative healing. Bromelain's reported efficacy in burn débridement and ischemia/reperfusion may also have positive applications in plastic surgery. Although Bromelain is widely used and generally considered to be a safe substance, more randomized, controlled clinical trials are necessary to further elucidate its clinical potential.
Bromelain: from production to commercialisation
J Sci Food Agric 2017 Mar;97(5):1386-1395.PMID:27790704DOI:10.1002/jsfa.8122.
Bromelain is a mixture of proteolytic enzymes found in pineapple (Ananas comosus) plants. It can be found in several parts of the pineapple plant, including the stem, fruit, leaves and peel. High demand for Bromelain has resulted in gradual increases in Bromelain production. These increases have led to the need for a Bromelain production strategy that yields more purified Bromelain at a lower cost and with fewer production steps. Previously, Bromelain was purified by conventional centrifugation, ultrafiltration and lyophilisation. Recently, the development of more modern purification techniques such as gel filtration, ion exchange chromatography, affinity chromatography, aqueous two-phase extraction and reverse micelle chromatography has resulted in increased industrial Bromelain production worldwide. In addition, recombinant DNA technology has emerged as an alternative strategy for producing large amounts of ultrapure Bromelain. An up-to-date compilation of data regarding the commercialisation of Bromelain in the clinical, pharmaceutical and industrial fields is provided in this review. © 2016 Society of Chemical Industry.