Bromobimane (Monobromobimane)
(Synonyms: 荧光专用试剂; Monobromobimane; (mBBr); 单溴二胺 ) 目录号 : GC30043
A thiol-reactive fluorogenic probe
Cas No.:71418-44-5
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal.1 Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides.2,3,4,5 The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.6
1.Kosower, N.S., Kosower, E.M., Newton, G.L., et al.Bimane fluorescent labels: Labeling of normal human red cells under physiological conditionsProc. Natl. Acad. Sci. USA76(7)3382-3386(1979) 2.Klingerman, C.M., Trushin, N., Prokopczyk, B., et al.H2S concentrations in the arterial blood during H2S administration in relation to its toxicity and effects on breathingAm. J. Physiol. Regul. Integr. Comp. Physiol.305(6)R630-R638(2013) 3.Rice, G.C., Bump, E.A., Shrieve, D.C., et al.Quantitative analysis of cellular glutathione by flow cytometry utilizing monochlorobimane: Some applications to radiation and drug resistance in vitro and in vivoCancer Res.46(12 Pt 1)6105-6110(1986) 4.Chen, Y.T., Collins, T.R.L., Guan, A., et al.Probing conformational changes in human DNA topoisomerase IIα by pulsed alkylation mass spectrometryJ. Biol. Chem.287(30)25660-25668(2012) 5.Cosstick, R., McLaughlin, L.W., and Eckstein, F.Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligaseNucleic Acids Res.12(4)1791-1810(2015) 6.Sabnis, R.W.Handbook of biological dyes and stains: Synthesis and industrial applications(2010)
|
Cell experiment: |
The stock mBBr solution is prepared by weight at 100-180 mM in acetonitrile. The stock solution of SBBr is prepared by weight at 50 mM in dimethyl sulfoxide (DMSO) or at 2 mM in the 20 mM Tris-methane sulfonate. The thiol is added to a final concentration of 1 mM to a solution of 2 mM mBBr or SBBr, 20 mM Tris-methane sulfonate, pH 8.0. The mixture is incubated in the dark for 15 min at room temperature and then methanesulfonic acid is added to 25 mM from a 5 M stock solution[2]. |
|
References: [1]. Kosower EM, et al. Bromobimane probes for thiols. Methods Enzymol. 1995;251:133-48. |
|
| Cas No. | 71418-44-5 | SDF | |
| 别名 | 荧光专用试剂; Monobromobimane; (mBBr); 单溴二胺 | ||
| Canonical SMILES | O=C1N(C(C(C)=C2C)=O)N2C(CBr)=C1C | ||
| 分子式 | C10H11BrN2O2 | 分子量 | 271.11 |
| 溶解度 | DMSO : ≥ 86.6 mg/mL (319.43 mM) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6885 mL | 18.4427 mL | 36.8854 mL |
| 5 mM | 0.7377 mL | 3.6885 mL | 7.3771 mL |
| 10 mM | 0.3689 mL | 1.8443 mL | 3.6885 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet