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BSA Control for Fatty Acid Complexes (5 mM)

目录号 : GC46102

BSA-脂肪酸复合物的对照

BSA Control for Fatty Acid Complexes (5 mM) Chemical Structure

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5ml
¥428.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

BSA control for BSA-fatty acid complexes (5 mM) is a fatty acid-free preparation of BSA intended for use as a control for BSA-fatty acid complexes containing 5 mM fatty acid (Cas No. GC46103). It can be used as a control for experiments using BSA-fatty acid complexes to deliver fatty acids to cells in culture for the purpose of monitoring fatty acid oxidation or similar processes in various cellular metabolic studies.[1,2,3] The BSA/BSA-FAs are acceptable for use with short term cell culture applications (acute treatment to 18 hours), however for long term 25+ hours the product can/should be filter sterilized using a 0.2 µm filter and sterile receptacle without affecting the performance.

Formulation: 0.8 mM BSA in 150 mM sodium chloride, pH 7.4

 

References:

[1]. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

[2]. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).

[3]. Bentebibel, A., Sebastián, D., Herrero, L., et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation. Biochemistry 45(14), 4339-4350 (2006).

Chemical Properties

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溶解度 储存条件 Store at -20°C, protect from light
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。
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Research Update

Liposomes as fatty acids carriers in isolated rat liver: effect on energy metabolism and on isolated mitochondria activity

MAGMA 2000 Feb;10(1):43-51.PMID:10697225DOI:10.1007/BF02613111.

The effects of fatty acids (FA)-carrier, egg-lecithin liposomes (LIPO) as alternative to BSA, on ATP, glycogen and glucose contents in isolated perfused liver of fed rats were non-invasively studied using 31P/13C nuclear magnetic resonance (NMR). Oxidative phosphorylation was studied in isolated mitochondria from the same liver consecutively to the NMR experiments. ATP content decreased slowly and ATP turnover was similar during the perfusion with saline solution (KHB) or LIPO. However, LIPO induced an enhancement of respiratory control ratio in isolated mitochondria. Tissue glycogen and glucose content decreased when FA (linoleate or linolenate) were perfused with defatted BSA (3%) or LIPO (600 mg/l) whereas glucose excretion level was unchanged and lactate excretion tended to increase, reflecting changes in the cytosolic redox state and/or an enhancement of glycolysis. Addition of FA (0.5 or 1.5 mM) to LIPO caused a dramatic fall in liver ATP, a mitochondrial uncoupling and an impairment of the phosphorylation activity. Perfusion with FA (1.5 mM) carried by BSA significantly increased the ATP degradation without change of mitochondrial function. Owing to the higher affinity of BSA than LIPO for FA, these latter could be more easily released from complex LIPO-FA, increasing their uncoupling effect. Hence, the FA concentrations have to be largely decreased from the above currently used concentrations to avoid this effect. It will then be possible to minimize the effector action of FA and to study their more specific metabolic function as fuel. It was concluded that LIPO were appropriate carriers to study the different metabolic effects of FA.