BSA-Palmitate Saturated Fatty Acid Complex (5 mM)
(Synonyms: BSA-PA) 目录号 : GC46103BSA-Palmitate Saturated Fatty Acid Complex由棕榈酸(PA)和牛血清白蛋白(BSA)组成,棕榈酸:BSA的摩尔比约为6:1,可用于将脂肪酸(FA)有效递送至培养物中的细胞,以在各种细胞代谢研究中监测脂肪酸氧化或类似过程。
Sample solution is provided at 25 µL, 10mM.
BSA-Palmitate Saturated Fatty Acid Complex is composed of palmitic acid (PA) and bovine serum albumin (BSA) in a molar ratio of approximately 6:1. It can be used to effectively deliver fatty acids (FA) to cells in culture to monitor fatty acid oxidation or similar processes in various cell metabolism studies[1-3]. BSA is one of the most abundant proteins in plasma and helps regulate the colloidal osmotic pressure of blood. It can be used in studies such as cell culture, immunochemistry, and protein quantification[4].
BSA/BSA-FA can be used for short-term cell culture applications (acute treatment up to 18h), but for long-term applications (more than 25h), the product should be filter-sterilized using a 0.2µm filter and sterile containers without affecting performance. For best results, it is recommended that this product be used in conjunction with the BSA Control of Fatty Acid Complex (Cat. No. GC46102).
References:
[1] Alsabeeh N, Chausse B, Kakimoto P A, et al. Cell culture models of fatty acid overload: Problems and solutions[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2018, 1863(2): 143-151.
[2] Wang D, Green M F, McDonnell E, et al. Oxygen flux analysis to understand the biological function of sirtuins[J]. Sirtuins: Methods and Protocols, 2013: 241-258.
[3] Bentebibel A, Sebastián D, Herrero L, et al. Novel effect of C75 on carnitine palmitoyltransferase I activity and palmitate oxidation[J]. Biochemistry, 2006, 45(14): 4339-4350.
[4] Jin M, Zhu S, Hou Y. Insight on serum albumin: from structure and biological properties to functional biomaterials for bone repair[J]. ACS Biomaterials Science & Engineering, 2023, 9(5): 2235-2250.
BSA-Palmitate Saturated Fatty Acid Complex由棕榈酸(PA)和牛血清白蛋白(BSA)组成,棕榈酸:BSA的摩尔比约为6:1,可用于将脂肪酸(FA)有效递送至培养物中的细胞,以在各种细胞代谢研究中监测脂肪酸氧化或类似过程[1-3]。BSA是血浆中最丰富的蛋白质之一,有助于调节血液的胶体渗透压,能够应用于细胞培养、免疫化学和蛋白质定量等研究[4]。
BSA/BSA-FA可用于短期细胞培养应用(急性处理至18小时),但是对于长期应用(25小时以上),应该使用0.2µm过滤器和无菌容器对产品进行过滤灭菌,而不会影响性能。为获得最佳结果,建议本品与脂肪酸复合物的BSA对照品(目录号GC46102)联合使用。
Cas No. | N/A | SDF | |
别名 | BSA-PA | ||
Canonical SMILES | N/A | ||
分子式 | N/A | 分子量 | N/A |
溶解度 | 储存条件 | Store at -20°C,protect from light | |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。