Buforin II
目录号 : GC68817Buforin II，来源于 buforin I，从亚洲蟾蜍 Bufo bufo gargarizans 胃中分离得到的蛋白，是一种高效的抗菌 (antimicrobial) 肽。Buforin II 对广谱的革兰氏阳性和革兰氏阴性细菌具有抗菌活性。
Cas No.:172998-24-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Buforin II, derived from buforin I, a protein isolated from the stomach of the Asian toad Bufo bufo gargarizans, is a potent antimicrobial peptide. Buforin II has antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria[1].
Buforin II inhibits A. baumanniiATCC 19606 and multiresistant strains with the similar MIC values of 8 mg/L[1].
Buforin II (intravenously a single dose, 1 mg/kg) has potent antibacterial activity that effectively reduces lethality and leads to a significant reduction in plasma endotoxin and cytokine concentrations in male Wistar rat model of sepsis[1].
| Animal Model: | Male Wistar rats weighing 200 to 300 g with A. baumannii ATCC 19606[1] |
| Dosage: | 1 mg/kg |
| Administration: | Intravenously a single dose |
| Result: | Reduced mortality by 40% in the treatment group and by 20% in the rifampicin (10 mg/kg) combination group. Reduced TNF, IL-6 and endotoxin plasma levels by 33%, 25% and 32%, respectively. |
| Animal Model: | Male Wistar rats weighing 200 to 300 g with the multiresistant strain[1] |
| Dosage: | 1 mg/kg |
| Administration: | Intravenously a single dose |
| Result: | Reduced mortality by 46.6% in the treatment group and by 20% in the rifampicin (10 mg/kg) combination group. Reduced TNF, IL-6 and endotoxin plasma levels by 46%, 20% and 28%, respectively. |
[1]. Oscar Cirioni, et al. Therapeutic efficacy of buforin II and rifampin in a rat model of Acinetobacter baumannii sepsis. Crit Care Med. 2009 Apr;37(4):1403-7.
| Cas No. | 172998-24-2 | SDF | Download SDF |
| 分子式 | C106H184N40O26 | 分子量 | 2434.85 |
| 溶解度 | H2O : 100 mg/mL (41.07 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.4107 mL | 2.0535 mL | 4.107 mL |
| 5 mM | 0.0821 mL | 0.4107 mL | 0.8214 mL |
| 10 mM | 0.0411 mL | 0.2054 mL | 0.4107 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。