C18 Ganglioside GM2-d7 (d18:1/18:0-d7) (ammonium salt, synthetic)
(Synonyms: C18 GM2-d7,Monosialoganglioside GM2-d7,N-omega-CD7-Octadecanoyl monosialoganglioside GM2,N-Stearoyl Monosialoganglioside GM2-d7) 目录号 : GC91845C18 Ganglioside GM2-d7 (d18:1/18:0-d7) is intended for use as an internal standard for the quantification of C18 ganglioside GM2 by GC- or LC-MS.
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
C18 Ganglioside GM2-d7 (d18:1/18:0-d7) is intended for use as an internal standard for the quantification of C18 ganglioside GM2 by GC- or LC-MS. C18 Ganglioside GM2 is a monosialylated ganglioside found in the mammalian brain where it is localized to the cell membrane.[1] It accumulates in Tay-Sachs and Sandhoff disease, which are neurodegenerative disorders characterized by deficiency of lysosomal β-hexosaminidase A and B, respectively.[2] Ganglioside GM2 disrupts the function of immune cells and, when used at a concentration of 25 µM, increases migration and invasion of SK-RC-45 cells with no effect on proliferation.[3] This product is fully synthetic and has no variations in the fatty acyl chain or sphingoid backbone. It is intended for use as an analytical standard for the quantification of C18 ganglioside GM2 by GC-MS.
References:
[1].Schnaar, R.L.Gangliosides of the vertebrate nervous systemJ. Mol. Biol.428(16)3325-3336(2016).
[2].Baek, R.C., Martin, D.R., Cox, N.R., et al.Comparative analysis of brain lipids in mice, cats, and humans with Sandhoff diseaseLipids44(3)197-205(2009).
[3].Kundu, M., Mahata, B., Banerjee, A., et al.Ganglioside GM2 mediates migration of tumor cells by interacting with integrin and modulating the downstream signaling pathwayBiochim. Biophys. Acta1863(7 Pt A)1472-1489(2016).
| Cas No. | N/A | SDF | Download SDF |
| 别名 | C18 GM2-d7,Monosialoganglioside GM2-d7,N-omega-CD7-Octadecanoyl monosialoganglioside GM2,N-Stearoyl Monosialoganglioside GM2-d7 | ||
| 分子式 | C67H113D7N3O26•NH4 | 分子量 | 1408.8 |
| 溶解度 | Methanol: Soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7098 mL | 3.5491 mL | 7.0982 mL |
| 5 mM | 0.142 mL | 0.7098 mL | 1.4196 mL |
| 10 mM | 0.071 mL | 0.3549 mL | 0.7098 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。