C20 Ceramide (d18:1/20:0)
(Synonyms: N-二十酰-D-神经鞘氨醇,Cer(d18:1/20:0)) 目录号 : GC43067C20 Ceramide (d18:1/20:0)是一种生物活性鞘脂，也是神经酰胺的一种，含有20个碳的脂肪酸链（20:0）和18个碳的鞘氨醇骨架（d18:1）。
Cas No.:7344-02-7
Sample solution is provided at 25 µL, 10mM.
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C20 Ceramide (d18:1/20:0) is a bioactive sphingolipid and a type of ceramide, containing a 20-carbon fatty acid chain (20:0) and an 18-carbon sphingosine backbone(d18:1).C20 Ceramide (d18:1/20:0) plays important roles in cellular signaling, particularly in apoptosis[1], cell cycle regulation, and stress responses[2].C20 Ceramide (d18:1/20:0) is commonly utilized in neurobiology research and cancer therpy[3], particularly in the study of its potential in Alzheimer's disease[4]. C20 Ceramide (d18:1/20:0) acts as a standard in quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry[5].
C20 Ceramide (d18:1/20:0) (2µM; 24h) treatment can induce apoptosis in primary cultured astrocytes from presenilin 1 (PS1) knock-in (PS1M146V) mice but not in wild-type astrocytes[6].
C20 Ceramide (d18:1/20:0) is elevated in presenilin 1 (PS1) knock-in (PS1M146V) mice brain[6].C20 Ceramide (d18:1/20:0) infusions of 100µM every other day for two weeks into the ventral hippocampus (VH) of adult male and female rats with implanted cannulas can trigger anhedonia-like behavior in the rats[7].
References:
[1]. Watts JD, Gu M, Patterson SD, Aebersold R, Polverino AJ. On the complexities of ceramide changes in cells undergoing apoptosis: lack of evidence for a second messenger function in apoptotic induction. Cell Death Differ. 1999 Feb;6(2):105-14. doi: 10.1038/sj.cdd.4400472. PMID: 10200556.
[2]. Kim HE, Grant AR, Simic MS, et al. Lipid Biosynthesis Coordinates a Mitochondrial-to-Cytosolic Stress Response. Cell. 2016 Sep 8;166(6):1539-1552.e16. doi: 10.1016/j.cell.2016.08.027. PMID: 27610574; PMCID: PMC5922983.
[3]. Sheridan M, Ogretmen B. The Role of Ceramide Metabolism and Signaling in the Regulation of Mitophagy and Cancer Therapy. Cancers (Basel). 2021 May 19;13(10):2475. doi: 10.3390/cancers13102475. PMID: 34069611; PMCID: PMC8161379.
[4]. Liu X, Jin Y, Cheng X, et al. The relevance between abnormally elevated serum ceramide and cognitive impairment in Alzheimer's disease model mice and its mechanism. Psychopharmacology (Berl). 2024 Mar;241(3):525-542. doi: 10.1007/s00213-024-06530-y. Epub 2024 Jan 26. PMID: 38277004.
[5]. Kasumov T, Huang H, Chung YM, et al. Quantification of ceramide species in biological samples by liquid chromatography electrospray ionization tandem mass spectrometry. Anal Biochem. 2010 Jun 1;401(1):154-61. doi: 10.1016/j.ab.2010.02.023. Epub 2010 Feb 21. PMID: 20178771; PMCID: PMC2872137.
[6]. Wang G, Silva J, Dasgupta S, Bieberich E. Long-chain ceramide is elevated in presenilin 1 (PS1M146V) mouse brain and induces apoptosis in PS1 astrocytes. Glia. 2008 Mar;56(4):449-56. doi: 10.1002/glia.20626. PMID: 18205190.
[7]. Sambolín-Escobales L, Feliciano-Quiñones A, Tirado-Castro L, et al. Infusion of C20:0 ceramide into ventral hippocampus triggers anhedonia-like behavior in female and male rats. Front Behav Neurosci. 2022 Aug 24;16:899627. doi: 10.3389/fnbeh.2022.899627. PMID: 36090653; PMCID: PMC9449580.
C20 Ceramide (d18:1/20:0)是一种生物活性鞘脂，也是神经酰胺的一种，含有20个碳的脂肪酸链（20:0）和18个碳的鞘氨醇骨架（d18:1）。C20 Ceramide (d18:1/20:0)在细胞信号传导中发挥重要作用，特别是在细胞凋亡[1]、细胞周期调控和应激反应中[2]。C20 Ceramide (d18:1/20:0)常用于神经生物学研究和癌症治疗[3]，尤其是研究其在阿尔茨海默病中的潜在作用[4]。C20 Ceramide (d18:1/20:0)可作为标准品，用于液相色谱电喷雾电离串联质谱法定量检测生物样本中神经酰胺[5]。
C20 Ceramide (d18:1/20:0)（2µM；24小时）处理可在早老素1（PS1）敲入（PS1M146V）小鼠来源的原代培养星形胶质细胞中诱导凋亡，但在野生型星形胶质细胞中则不能[6]。
在早老素1（PS1M146V）小鼠的大脑中，C20 Ceramide (d18:1/20:0)水平升高[6]。通过植入导管，连续两周每两天向成年雄性和雌性大鼠的腹侧海马（VH）中注入100µM的C20 Ceramide (d18:1/20:0)可在大鼠中引发类似快感缺失的行为[7]。
| Cell experiment [1]: | |
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Cell lines |
Astrocytes from presenilin 1(PS1) mice |
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Preparation Method |
Primary cultured astrocytes from presenilin 1(PS1) mice were incubated for 24h with 2µM C20 Ceramide (d18:1/20:0) , or vehicle (1:1000 ethanol/2% dodecane). Cells were fixed and stained for Hoechst was performed. Quantification of apoptosis induced by ceramide by counting cells with condensed nuclei. More than 200 cells were counted for each experiment. |
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Reaction Conditions |
2µM; 24h |
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Applications |
C20 Ceramide (d18:1/20:0) can induce apoptosis of primary cultured astrocytes from presenilin 1(PS1) mice. |
| Animal experiment [2]: | |
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Animal models |
Sprague Dawley rats |
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Preparation Method |
Adult male and female rats were implanted with cannulas in the ventral hippocampus (VH) and infused with 100µM C20 Ceramide (d18:1/20:0) or vehicle every other day for 2 weeks. Anhedonia-like behavior was assessed with the sucrose preference test 24h after each VH infusion of C20 Ceramide (d18:1/20:0). |
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Dosage form |
100µM; Every other day; 2 weeks; i.p. |
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Applications |
Localized ventral hippocampus infusions of C20 Ceramide (d18:1/20:0) can induce anhedonia-like behavior in male and female rats |
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References: |
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| Cas No. | 7344-02-7 | SDF | |
| 别名 | N-二十酰-D-神经鞘氨醇,Cer(d18:1/20:0) | ||
| 化学名 | N-[2R-hydroxy-1S-(hydroxymethyl)-3E-heptadecen-1-yl]-eicosanamide | ||
| Canonical SMILES | CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCCCCCCCCCCCCCCC)=O)CO | ||
| 分子式 | C38H75NO3 | 分子量 | 594 |
| 溶解度 | Ethanol: 6mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6835 mL | 8.4175 mL | 16.835 mL |
| 5 mM | 0.3367 mL | 1.6835 mL | 3.367 mL |
| 10 mM | 0.1684 mL | 0.8418 mL | 1.6835 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
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