C22 Ceramide (d18:1/22:0)
(Synonyms: C22 Ceramide；Cer(d18:1/22:0)；Ceramide (d18:1/22:0)) 目录号 : GC43069C22 Ceramide (d18:1/22:0)是一种从海绵Haliclona koremella中分离的含有山萮酸的长链神经酰胺，是一种内源性生物活性鞘脂。
Cas No.:27888-44-4
Sample solution is provided at 25 µL, 10mM.
C22 Ceramide (d18:1/22:0) is a long-chain ceramide containing behenic acid isolated from the sea sponge Haliclona koremella and is an endogenous bioactive sphingolipid. C22 Ceramide (d18:1/22:0) is widely used to study lipid-mediated signal transduction mechanisms that affect cell homeostasis, apoptosis, and differentiation[1][2]. C22 Ceramide (d18:1/22:0) can also be used as a standard for measuring ceramide concentration in samples by high-performance liquid chromatography/tandem mass spectrometry (LC/MS2) and as a standard for the quantification of sphingolipid metabolites[3][4].
C22 Ceramide (d18:1/22:0) (40nmol; 15min) reduces the propensity of C16-ceramide channel formation in isolated rat liver mitochondria and in liposomes[1]. C22 ceramide (d18:1/22:0) can inhibit the mitochondrial to cytoplasmic stress response induced by hsp-6 RNAi[2]. C22 Ceramide (d18:1/22:0) (0.2µM; 48h) activated miR-199a-3p and inhibited mammalian target of rapamycin(mTOR) level, thus activating the mTOR-ULK1 signaling pathway by promoting the expression of unc-51-like autophagy activating kinase 1 (ULK1), B-cell lymphoma-2 (Bcl-2), Beclin-1, autophagy-related gene 5 (ATG5), and microtubule-associated protein light chain 3 (LC3-II) and degrading p62[5].
References:
[1]. Stiban J, Perera M. Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2015, 1848(2): 561-567.
[2]. Kim H E, Grant A R, Simic M S, et al. Lipid biosynthesis coordinates a mitochondrial-to-cytosolic stress response[J]. Cell, 2016, 166(6): 1539-1552. e16.
[3]. Kauhanen D, Sysi-Aho M, Koistinen K M, et al. Development and validation of a high-throughput LC–MS/MS assay for routine measurement of molecular ceramides[J]. Analytical and bioanalytical chemistry, 2016, 408: 3475-3483.
[4]. Aslan M, Afşar E, Kırımlıoglu E, et al. Antiproliferative effects of thymoquinone in MCF-7 breast and HepG2 liver cancer cells: possible role of ceramide and ER stress[J]. Nutrition and Cancer, 2021, 73(3): 460-472.
[5]. Wan J, Zhang S, Li G, et al. Ceramide Ehux-C22 Targets the miR-199a-3p/mTOR Signaling Pathway to Regulate Melanosomal Autophagy in Mouse B16 Cells[J]. International Journal of Molecular Sciences, 2024, 25(15): 8061.
C22 Ceramide (d18:1/22:0)是一种从海绵Haliclona koremella中分离的含有山萮酸的长链神经酰胺，是一种内源性生物活性鞘脂。C22 Ceramide (d18:1/22:0)广泛用于细胞稳态、凋亡和分化的脂质介导信号转导机制的研究[1][2]。C22 Ceramide (d18:1/22:0)还可以用作高效液相色谱/串联质谱(LC/MS2)测量样品中神经酰胺浓度的标准品，以及定量鞘脂代谢物的标准品[3][4]。
C22 Ceramide (d18:1/22:0)（40nmol；15min）降低了分离的大鼠肝线粒体和脂质体中C16-神经酰胺通道的形成[1]。C22 Ceramide (d18:1/22:0)能够抑制hsp-6 RNAi诱导的线粒体到细胞质的应激反应[2]。C22 Ceramide (d18:1/22:0)（0.2µM; 48h）激活miR-199a-3p并抑制哺乳动物雷帕霉素靶蛋白（mTOR）水平，从而通过促进unc-51样自噬激活激酶1（ULK1）、B细胞淋巴瘤-2（Bcl-2）、Beclin-1、自噬相关基因5（ATG5）和微管相关蛋白轻链3（LC3-II）的表达并降解p62来激活mTOR-ULK1信号通路[5]。
| Cell experiment [1]: | |
Cell lines | B16 cells |
Preparation Method | B16 cells were cultured with RPMI 1640 medium provided with 10% FBS and 1% PS (5% CO2, 37°C) overnight. Dissolved in DMSO and MTBE (1:1), 2µM C22 Ceramide (d18:1/22:0) was added to the cell. |
Reaction Conditions | 0.2µM; 48h |
Applications | C22 Ceramide (d18:1/22:0) activated miR-199a-3p and inhibited mammalian target of rapamycin(mTOR) level, thus activating the mTOR-ULK1 signaling pathway by promoting the expression of unc-51-like autophagy activating kinase 1 (ULK1), B-cell lymphoma-2 (Bcl-2), Beclin-1, autophagy-related gene 5 (ATG5), and microtubule-associated protein light chain 3 (LC3-II) and degrading p62. |
References: | |
| Cas No. | 27888-44-4 | SDF | |
| 别名 | C22 Ceramide；Cer(d18:1/22:0)；Ceramide (d18:1/22:0) | ||
| 化学名 | N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-docosanamide | ||
| Canonical SMILES | OC[C@H](NC(CCCCCCCCCCCCCCCCCCCCC)=O)[C@H](O)/C=C/CCCCCCCCCCCCC | ||
| 分子式 | C40H79NO3 | 分子量 | 622.1 |
| 溶解度 | 0.15mg/ml in DMF | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6075 mL | 8.0373 mL | 16.0746 mL |
| 5 mM | 0.3215 mL | 1.6075 mL | 3.2149 mL |
| 10 mM | 0.1607 mL | 0.8037 mL | 1.6075 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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