C6 Ceramide (d18:1/6:0)
(Synonyms: N-己酰基-D-神经鞘氨醇,N-hexanoyl-D-erythro-sphingosine) 目录号 : GC11079C6 Ceramide (d18:1/6:0)是一种短链的、细胞可透过的神经酰胺类似物，在临床前研究中用于模拟内源性神经酰胺的作用。
Cas No.:124753-97-5
Sample solution is provided at 25 µL, 10mM.
C6 Ceramide (d18:1/6:0) is a short-chain, cell-permeable analog of ceramide that has been utilized in preclinical studies to mimic the effects of endogenous ceramide[1]. C6 Ceramide (d18:1/6:0) can induce cell death by both apoptosis and necrosis[2]. C6 Ceramide (d18:1/6:0) is primarily used in research areas such as cancer biology[3][4].
C6 Ceramide (d18:1/6:0) (25µM; 24h) treatment strongly reduced cell viability of CTCL cell lines and induced cell death via apoptosis and necrosis[5]. In MDA-231 and MCF-7 cells, treatment with C6 Ceramide (d18:1/6:0) at 10μg/mL for 72 hours dramatically enhances docetaxel-induced cytotoxicity[6].
C6 Ceramide (d18:1/6:0) (60mg/kg; 15d; i.p.) treatment effectively suppresses the growth of CHMp xenograft tumors in mice[7]. C6 Ceramide (d18:1/6:0) (10mg/kg; 6d; i.p.) combined with Trichostatin-A treatment showed significant tumor growth inhibition, increased survival rate and reduced tumor size in mice xenograft pancreatic and ovarian cancer models[8].
References:
[1]. Morad SA, Ryan TE, Neufer PD, et al. Ceramide-tamoxifen regimen targets bioenergetic elements in acute myelogenous leukemia. J Lipid Res. 2016 Jul;57(7):1231-42. doi: 10.1194/jlr.M067389. Epub 2016 May 2. PMID: 27140664; PMCID: PMC4918852.
[2]. Gentil B, Grimot F, Riva C. Commitment to apoptosis by ceramides depends on mitochondrial respiratory function, cytochrome c release and caspase-3 activation in Hep-G2 cells. Mol Cell Biochem. 2003 Dec;254(1-2):203-10. doi: 10.1023/a:1027359832177. PMID: 14674699.
[3]. Flowers M, Fabriás G, Delgado A, et al. C6-ceramide and targeted inhibition of acid ceramidase induce synergistic decreases in breast cancer cell growth. Breast Cancer Res Treat. 2012 Jun;133(2):447-58. doi: 10.1007/s10549-011-1768-8. Epub 2011 Sep 21. PMID: 21935601.
[4]. Fillet M, Bentires-Alj M, Deregowski V, et al. Mechanisms involved in exogenous C2- and C6-ceramide-induced cancer cell toxicity. Biochem Pharmacol. 2003 May 15;65(10):1633-42. doi: 10.1016/s0006-2952(03)00125-4. PMID: 12754099.
[5]. Wilhelm R, Eckes T, Imre G, et al. C6 Ceramide (d18:1/6:0) as a Novel Treatment of Cutaneous T Cell Lymphoma. Cancers (Basel). 2021 Jan 13;13(2):270. doi: 10.3390/cancers13020270. PMID: 33450826; PMCID: PMC7828274.
[6]. Yang L, Zheng LY, Tian Y, et al. C6 ceramide dramatically enhances docetaxel-induced growth inhibition and apoptosis in cultured breast cancer cells: a mechanism study. Exp Cell Res. 2015 Mar 1;332(1):47-59. doi: 10.1016/j.yexcr.2014.12.017. Epub 2015 Jan 6. PMID: 25576381.
[7]. Liu J, Zhao F, Zhang Y, et al. C6 Ceramide Inhibits Canine Mammary Cancer Growth and Metastasis by Targeting EGR3 through JAK1/STAT3 Signaling. Animals (Basel). 2024 Jan 27;14(3):422. doi: 10.3390/ani14030422. PMID: 38338065; PMCID: PMC10854580.
[8]. Zhu QY, Wang Z, Ji C, et al. C6-ceramide synergistically potentiates the anti-tumor effects of histone deacetylase inhibitors via AKT dephosphorylation and α-tubulin hyperacetylation both in vitro and in vivo. Cell Death Dis. 2011 Jan 27;2(1):e117. doi: 10.1038/cddis.2010.96. PMID: 21368888; PMCID: PMC3077291.
C6 Ceramide (d18:1/6:0)是一种短链的、细胞可透过的神经酰胺类似物，在临床前研究中用于模拟内源性神经酰胺的作用[1]。C6 Ceramide (d18:1/6:0)可以通过凋亡和坏死诱导细胞死亡[2]。C6 Ceramide (d18:1/6:0) 主要用于癌症生物学等研究领域[3][4]。
C6 Ceramide (d18:1/6:0) (25µM; 24h)处理显著降低了CTCL细胞系的细胞活力，并通过凋亡和坏死诱导细胞死亡[5]。在MDA-231和MCF-7细胞中，使用10μg/mL的C6 Ceramide (d18:1/6:0)处理72小时显著增强了多西他赛引起的细胞毒性[6]。
C6 Ceramide (d18:1/6:0) (60mg/kg; 15天; 腹腔注射) 处理有效抑制了小鼠体内CHMp异种移植肿瘤的生长[7]。C6 Ceramide (d18:1/6:0) (10mg/kg; 6天; 腹腔注射) 联合曲古抑菌素A的治疗在小鼠异种移植的胰腺癌和卵巢癌模型中有显著的肿瘤生长抑制效果，增加了生存率并减小了肿瘤体积[8]。
| Cell experiment [1]: | |
Cell lines | HaCaT cells |
Preparation Method | HaCaT cells were treated with 1, 5, 10, 25, 50, and 100µM C6 Ceramide (d18:1/6:0) for 24h. Then Cell viability was determined by MTS assay. To measure cell viability, 3×104 HaCaT cells were seeded in 96-well plates in 100µL volume and 10µL MTS was added and incubated at 37◦C for 1h. The absorbance at 490nm was recorded using the microplate reader. |
Reaction Conditions | 1, 5, 10, 25, 50, 100µM; 24h |
Applications | C6 Ceramide (d18:1/6:0) treatment can significantly reduce the cell viability of HaCaT cells. |
| Animal experiment [2]: | |
Animal models | Primary tumor model Mouse |
Preparation Method | Primary tumor-bearing mouse were treatment with 60 mg/kg C6 Ceramide (d18:1/6:0) for 15 days, the tumor volume were measure at the end of the experiment. |
Dosage form | 60mg/kg/d; 15d; i.p. |
Applications | C6 Ceramide (d18:1/6:0) treatment effectively suppresses the growth of CHMp xenograft tumors in vivo. |
References: | |
| Cas No. | 124753-97-5 | SDF | |
| 别名 | N-己酰基-D-神经鞘氨醇,N-hexanoyl-D-erythro-sphingosine | ||
| 化学名 | N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-hexanamide | ||
| Canonical SMILES | CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCC)=O)CO | ||
| 分子式 | C24H47NO3 | 分子量 | 397.6 |
| 溶解度 | 20mg/mL in DMSO, 22mg/mL in DMF, 33mg/mL in Ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5151 mL | 12.5755 mL | 25.1509 mL |
| 5 mM | 0.503 mL | 2.5151 mL | 5.0302 mL |
| 10 mM | 0.2515 mL | 1.2575 mL | 2.5151 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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