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CA13 Human

目录号 : GP21460

Carbonic Anhydrase XIII Human Recombinant

CA13 Human Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥72,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

CA13 HumanRecombinant produced in E.Coli is a single, non-glycosylated polypeptide chaincontaining 285 amino acids (1-262a.a) and having a molecular mass of 31.8kDa.CA13 is fused to a 23amino acid His-tag at N-terminus & purified by proprietary chromatographictechniques.

Product Data

Purity Greater than 90.0% asdetermined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered clear solution. Shipping Condition Shipped with Ice Packs.
Synonyms Carbonic AnhydraseXIII; Carbonate Dehydratase XIII; EC 4.2.1.1; CA-XIII;Carbonic; Anhydrase 13; CAXIII.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSPMGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSFNVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAHEPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLESVTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH.
Stability Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Biological Activity Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Formulation CA13 proteinsolution (0.5mg/ml) containing Phosphate buffered saline (pH7.4),10% glycerol and 1mM DTT.

Introduction

Carbonic Anhydrase XIII, also known as CA13 is a member of thealpha-carbonic anhydrase family, which catalyzes the rapid interconversion ofcarbon dioxide and water to bicarbonate andprotons, a reversible reaction which occurs relatively slowly in the absence ofcatalyst. Furthermore, the active site of nearly allcarbonic anhydrases contains a zinc ion; they have been classified asmetalloenzymes. At least five distinct CA families(?, ?, ?, ? and ?) have been found. These families have no significant a.asequence resemblance and in just about all cases are consideredto be an example of convergent evolution. The ?-CAs have been demonstrated inhumans.

Biological Activity

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

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