Caffeic Acid Phenethyl Ester
(Synonyms: 咖啡酸苯乙酯) 目录号 : GC10692
A potent inhibitor of NF-κB activation
Cas No.:104594-70-9
Sample solution is provided at 25 µL, 10mM.
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Caffeic acid phenethyl ester (CAPE) is an inhibitor of NF-κB activation [1].
CAPE derives from honeybee propolis and has several biological activities. It is found to be an inhibitor of NF-κB activation. In U937 cells, CAPE inhibits the TNF-dependent activation of NF-κB dose-dependently. The maximum effect occurs at dose of 25μg/ml. CAPE also inhibits NF-κB activation induced by phorbol ester, ceramide, okadaic acid and hydrogen peroxide. It is reported that CAPE prevents NF-κB from binding to DNA without affecting other transcription factors. CAPE also has angiogenesis efficacy. It shows inhibition effect on the formation of capillary-like tubes. Besides, it reduces the production of VEGF both in CT26 cell culture and in animal model. In addition, CAPE effectively prevents CT26 cells from secreting MMP-2 and MMP-9 [1, 2].
References:
[1] Natarajan K, Singh S, Burke TR Jr, Grunberger D, Aggarwal BB. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9090-5.
[2] Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Shiao MS, Chen YJ. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem. 2003 Dec 31;51(27):7907-12.
| Cell experiment [1]: | |
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Cell lines |
The human histiocytic cell line U937 cells |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
0, 5, 10, 15, 25 and 30 μg/ml; 2 h |
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Applications |
In U937 cells, Caffeic Acid Phenethyl Ester (CAPE) inhibited the TNF-dependent activation of NF-KB in a dose-dependent manner, with maximum effect occurring at 25 ug/ml. CAPE (25 ug/ml) also blocked NF-KB activation induced by Phorbol Ester, Ceramide, Okadaic Acid, and Hydrogen Peroxide. |
| Animal experiment [2]: | |
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Animal models |
CT26-bearing BALB/c male mice with pulmonary metastases |
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Dosage form |
10 mg/kg/day, intraperitoneal injection |
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Application |
In CT26-bearing BALB/c male mice with pulmonary metastases, CAPE decreased tumor colonization in the lung. Mice treated with CAPE survived longer than untreated controls. CAPE reduced plasma VEGF levels by 53.2%. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Natarajan K, Singh S, Burke TR Jr, Grunberger D, Aggarwal BB. Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc Natl Acad Sci U S A. 1996 Aug 2;93(17):9090-5. [2] Liao HF, Chen YY, Liu JJ, Hsu ML, Shieh HJ, Liao HJ, Shieh CJ, Shiao MS, Chen YJ. Inhibitory effect of caffeic acid phenethyl ester on angiogenesis, tumor invasion, and metastasis. J Agric Food Chem. 2003 Dec 31;51(27):7907-12. | |
| Cas No. | 104594-70-9 | SDF | |
| 别名 | 咖啡酸苯乙酯 | ||
| 化学名 | 2-phenylethyl (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | ||
| Canonical SMILES | C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O | ||
| 分子式 | C17H16O4 | 分子量 | 284.31 |
| 溶解度 | ≥ 28.4mg/mL in DMSO | 储存条件 | Store at -20° C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL |
| 5 mM | 0.7035 mL | 3.5173 mL | 7.0346 mL |
| 10 mM | 0.3517 mL | 1.7586 mL | 3.5173 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet