Calcifediol monohydrate

Name: Calcifediol monohydrate
SKU: GC16720
Availability: InStock
Rating: 5 (30 reviews)

(Synonyms: 骨化二醇一水合物,25-hydroxy Vitamin D3 monohydrate) 目录号 : GC16720

Calcifediol monohydrate (25-hydroxy Vitamin D3 monohydrate; 骨化二醇)是维生素D受体（VDR）的配体，具有基因调控和抗增殖特性，可作为维生素D补充剂和VDR拮抗剂。

Calcifediol monohydrate Chemical Structure

Cas No.:63283-36-3

规格价格库存购买数量

5mg	¥630.00	现货
25mg	¥2,363.00	现货
100mg	¥7,581.00	现货
500mg	¥24,098.00	现货

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Sample solution is provided at 25 µL, 10mM.

GlpBio产品文献引用

Nature
610.7931 (2022): 366-372

Nature
618, 1017–1023 (2023)

Cell
183.7 (2020): 1867-1883

Cell Research
31, 1291–1307 (2021)

Cell Research
33, 273–287 (2023)

Cell Research
33, 546–561 (2023)

Molecular Cancer
21.1 (2022): 1-17

Molecular Cancer
21.1 (2022): 1-18

Cancer Cell
39 (2021): 1-16

Cell Host Microbe
30.8 (2022): 1124-1138

Cell Host Microbe
31.5 (2023): 766-780

Cell Host Microbe
31.3 (2023): 418-43

Cell Metabolism
34.2 (2022): 240-255

Ann Rheum Dis
82(4): 533-545 (2023)

Cell Mol Immunol
20, 264–276 (2023)

Adv Funct Mater
33.35 (2023): 2214998

产品文档

Quality Control & SDS

View current batch:

Purity: >99.00%

实验参考方法

Cell experiment [1]:
Cell lines	Neuroblastoma cells
Preparation Method	Cells were treated with Calcifediol monohydrate in a final concentration of 100nM or solvent control (EtOH).
Reaction Conditions	100nM; 24h
Applications	Calcifediol monohydrate decreased amyloid-β (Aβ)-production and increased Aβ-degradation in neuroblastoma cells.
Animal experiment [2]:
Animal models	Atopic dermatitis (AD) mice model (Female BALB/c mice)
Preparation Method	The AD mice model was induced by using DNCB. 1% DNCB was applied once a day to the ears (5μL) of AD-induced mice on days 6, 10, 14, and 18. The treatment was started on the day of challenge (day 6) with an intraperitoneal injection of 100µL Calcifediol monohydrate(5µg/kg). Each dose was given once every two days for a total of seven doses.
Dosage form	5µg/kg, 100µL; 7 times; i.p
Applications	Calcifediol monohydrate treatment reduces inflammatory infiltration and edema in the ears of AD mice.
References: [1]. Grimm MOW, Thiel A, et,al. Vitamin D and Its Analogues Decrease Amyloid-β (Aβ) Formation and Increase Aβ-Degradation. Int J Mol Sci. 2017 Dec 19;18(12):2764. doi: 10.3390/ijms18122764. PMID: 29257109; PMCID: PMC5751363. [2]. Lu R, Peng Z, et,al. Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function. Int Immunopharmacol. 2023 Sep;122:110558. doi: 10.1016/j.intimp.2023.110558. Epub 2023 Jun 30. PMID: 37393836.

产品描述

Calcifediol monohydrate (25-hydroxy Vitamin D3 monohydrate; 25(OH)D3) is a potent ligand for the vitamin D receptor (VDR) with gene regulatory and anti-proliferative properties, and functions as a vitamin D supplement and a VDR antagonist. As a precursor hormone in the vitamin D endocrine system (VDES), calcifediol undergoes hydroxylation in the liver to form the active hormone, calcitriol. This form of vitamin D can rapidly increase serum vitamin D levels^[1-3].

Calcifediol monohydrate (100nM; 24h) decreased amyloid-β (Aβ)-production and increased Aβ-degradation in neuroblastoma cells. Effects were mediated by affecting the Aβ-producing enzymes BACE1 and γ-secretase ^[4]. Calcifediol monohydrate(10⁻⁵M) can induce a rapid increase in Ca²⁺ levels in hADMSCs cells in vitro ^[5].

Calcifediol monohydrate (5µg/kg, 100µL; 7 times; i.p)significantly protected mice against DNCB-induced AD. Calcifediol monohydrate may reduce inflammatory cell infiltration and chemokines by inhibiting the phosphorylation of STAT3 and may restore skin barrier function through the downregulation of AQP3 protein expression and inhibition of cell proliferation in In AD mouse model ^[6]. After viral attack, mice supplemented with Calcifediol monohydrate(125mg/kg; p.o; 7 weeks) stimulated the production of both anti-inflammatory and pro-inflammatory cytokines^[7].

References:
[1]. Castoldi A, Herr C, et,al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm. 2017 Sep;118:62-67. doi: 10.1016/j.ejpb.2016.11.026. Epub 2016 Nov 22. PMID: 27888144.
[2]. Zheng W, Duan B, et,al. Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β-catenin signaling. Biosci Rep. 2018 Dec 7;38(6):BSR20180595. doi: 10.1042/BSR20180595. PMID: 30314996; PMCID: PMC6435469.
[3]. Lou YR, Molnár F, Peräkylä M, Qiao S, Kalueff AV, St-Arnaud R, Carlberg C, Tuohimaa P. 25-Hydroxyvitamin D(3) is an agonistic vitamin D receptor ligand. J Steroid Biochem Mol Biol. 2010 Feb 15;118(3):162-70. doi: 10.1016/j.jsbmb.2009.11.011. Epub 2009 Nov 26. PMID: 19944755.
[4]. Grimm MOW, Thiel A, Lauer AA, Winkler J, Lehmann J, Regner L, Nelke C, Janitschke D, Benoist C, Streidenberger O, Stötzel H, Endres K, Herr C, Beisswenger C, Grimm HS, Bals R, Lammert F, Hartmann T. Vitamin D and Its Analogues Decrease Amyloid-β (Aβ) Formation and Increase Aβ-Degradation. Int J Mol Sci. 2017 Dec 19;18(12):2764. doi: 10.3390/ijms18122764. PMID: 29257109; PMCID: PMC5751363.
[5]. Donati S, Palmini G, Romagnoli C, Aurilia C, Miglietta F, Falsetti I, Marini F, Zonefrati R, Galli G, Marcucci G, Iantomasi T, Brandi ML. In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells. Nutrients. 2021 Nov 25;13(12):4227. doi: 10.3390/nu13124227. PMID: 34959778; PMCID: PMC8707877.
[6]. Lu R, Peng Z, Lian P, Wazir J, Gu C, Ma C, Wei L, Li L, Pu W, Liu J, Wang H, Su Z. Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function. Int Immunopharmacol. 2023 Sep;122:110558. doi: 10.1016/j.intimp.2023.110558. Epub 2023 Jun 30. PMID: 37393836.
[7]. Hayashi H, Okamatsu M, et,al. Oral Supplementation of the Vitamin D Metabolite 25(OH)D3 Against Influenza Virus Infection in Mice. Nutrients. 2020 Jul 5;12(7):2000. doi: 10.3390/nu12072000. PMID: 32635656; PMCID: PMC7400405.

Calcifediol monohydrate (25-hydroxy Vitamin D3 monohydrate; 骨化二醇)是维生素D受体（VDR）的配体，具有基因调控和抗增殖特性，可作为维生素D补充剂和VDR拮抗剂。作为维生素D内分泌系统(VDES)的前体激素，Calcifediol在肝脏中发生羟基化作用，形成活性激素骨化三醇。这种形式的维生素D可以迅速提高血清维生素D水平^[1-3]。

Calcifediol monohydrate (100nM; 24h)在神经母细胞瘤细胞中减少了淀粉样β(Aβ)产生并增加了Aβ的降解。其作用是通过影响Aβ生成酶BACE1和γ-分泌酶介导的^[4]。Calcifediol monohydrate(10⁻⁵M)可以在体外诱导人脂肪来源的间充质干细胞（hADMSCs）中钙离子(Ca²⁺)水平的快速升高^[5]。

Calcifediol monohydrate (5µg/kg, 100µL; 7 times; i.p)显著保护特应性皮炎小鼠免受DNCB诱导的特应性皮炎(AD)影响。Calcifediol monohydrate可能通过抑制STAT3的磷酸化减少炎症细胞的浸润和趋化因子的生成，并通过下调AQP3蛋白表达和抑制细胞增殖来恢复皮肤屏障功能^[6]。在病毒攻击后，补充Calcifediol monohydrate(125mg/kg; p.o; 7 weeks)的小鼠能够刺激抗炎和促炎细胞因子的产生^[7]。

Chemical Properties

Cas No.	63283-36-3	SDF
别名	骨化二醇一水合物,25-hydroxy Vitamin D3 monohydrate
化学名	(1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol;hydrate
Canonical SMILES	CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C.O
分子式	C₂₇H₄₆O₃	分子量	418.65
溶解度	DMSO : 50 mg/mL (119.43 mM);Soluble in Chloroform	储存条件	Store at -20°C, protect from light
General tips	请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。
Shipping Condition	评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。

溶解性数据

制备储备液
		1 mg	5 mg	10 mg
	1 mM	2.3886 mL	11.9432 mL	23.8863 mL
	5 mM	0.4777 mL	2.3886 mL	4.7773 mL
	10 mM	0.2389 mL	1.1943 mL	2.3886 mL

摩尔浓度计算器
稀释计算器
分子量计算器

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动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % saline

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计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系GLPBIO）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。