Calmodulin-Dependent Protein Kinase II 290-309
目录号 : GC31170
A CaMKII inhibitor
Cas No.:115044-69-4
Sample solution is provided at 25 µL, 10mM.
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Calmodulin-dependent protein kinase II (290-309) is a synthetic peptide derived from the rat brain protein sequence that contains the calmodulin binding domain.1,2 It inhibits calcium/calmodulin-dependent protein kinase II (CaMKII) with an IC50 value of 52 nM and CaMKII-dependent phosphodiesterase activity (IC50 = 1.1 nM). Calmodulin-dependent protein kinase II (290-309) has been used in the study of CaM binding, autophosphorylation, and dynamics.3,4
1.Payne, E.M., Fong, Y.-L., Ono, T., et al.Calcium/Calmodulin-dependent Protein Kinase IIJ. Biol. Chem.263(15)7190-7195(1988) 2.Lin, C.R., Kapiloff, M.S., Durgerian, S., et al.Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinaseProc. Natl. Acad. Sci. USA84(16)5962-5966(1987) 3.Wyttenbach, T., Grabenauer, M., Thalassinos, K., et al.The effect of calcium ions and peptide ligands on the relative stabilities of the calmodulin dumbbell and compact structuresJ. Phys. Chem. B.114(1)437-447(2010) 4.Colbran, R.J., and Soderling, T.R.Calcium/calmodulin-independent autophosphorylation sites of calcium/calmodulin-dependent protein kinase II. Studies on the effect of phosphorylation of threonine 305/306 and serine 314 on calmodulin binding using synthetic peptidesJ. Biol. Chem.265(19)11213-11219(1990)
Kinase experiment: | Ca2+/CaM-dependent phosphodiesterase is assayed at 30°C. The reaction mixture contains 40 mM Tris-HCl, pH 8.0, 5 mM magnesium acetate, 1 mM calcium chloride, 30 μM cGMP, 0.15 pCi of [3H]cGMP, 1 mM dithiothreitol, 20% glycerol, 0.64 mg/mL bovine serum albumin, 2.38 nM CaM, 50 pM CaM-deficient phosphodiesterase, and various concentrations of Calmodulin-Dependent Protein Kinase II (290-309). Following preincubation of the reaction mixture at 30°C, the reaction is initiated by the addition of substrate. After 50 min the reaction is terminated by boiling. Conditions are selected in which the reactions are linear with respect to time[1]. |
References: [1]. Payne ME, et al. Calcium/calmodulin-dependent protein kinase II. Characterization of distinct calmodulin binding and inhibitory domains. J Biol Chem. 1988 May 25;263(15):7190-5. | |
| Cas No. | 115044-69-4 | SDF | |
| Canonical SMILES | Leu-Lys-Lys-Phe-Asn-Ala-Arg-Arg-Lys-Leu-Lys-Gly-Ala-Ile-Leu-Thr-Thr-Met-Leu-Ala | ||
| 分子式 | C103H185N31O24S | 分子量 | 2273.83 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.4398 mL | 2.1989 mL | 4.3979 mL |
| 5 mM | 0.088 mL | 0.4398 mL | 0.8796 mL |
| 10 mM | 0.044 mL | 0.2199 mL | 0.4398 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet