Carbacyclin (Carbaprostacyclin)
(Synonyms: Carbaprostacyclin; Carba-PGI2) 目录号 : GC32487
A stable analog of PGI2
Cas No.:69552-46-1
Sample solution is provided at 25 µL, 10mM.
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Carbaprostacyclin is a stable analog of PGI2. When infused in rabbits or dogs, it inhibits ex vivo platelet aggregation, but the effect persists only 10 minutes after termination of the infusion. This implies rapid metabolic inactivation of carbaprostacyclin.1 Carbaprostacyclin inhibits platelet aggregation with 10% of the molar potency exhibited by PGI2.1,2 The ED50 of carbaprostacyclin for the in vitro inhibition of ADP-
1.Whittle, B.J.R., Moncada, S., Whiting, F., et al.Carbacyclin — a potent stable prostacyclin analogue for the inhibition of platelet aggregationProstaglandins19(4)605-627(1980) 2.Aiken, J.W., and Shebuski, R.J.Comparison in anesthetized dogs of the anti-aggregatory and hemodynamic effects of prostacyclin and a chemically stable prostacyclin analog, 6a-carba-PGI2 (carbacyclin)Prostaglandins19(4)629-643(1980) 3.Adaikan, P.G., Karim, S.M.M., and Lau, L.C.Platelet and other effects of carbaprostacyclin - a stable prostacyclin analogueProstaglandins Med.5(4)307-320(1980) 4.Aubert, J., Ailhaud, G., and Negrel, R.Evidence for a novel regulatory pathway activated by (carba)prostacyclin in preadipose and adipose cellsFEBS Lett.397(1)117-121(1996)
Cell experiment: | Primary cultures of neonatal rat cardiomyocytes are prepared from the ventricles of 1-day-old Wistar rats, and are seeded at a density of 4 × 105/6-well plastic plates, 9 × 105/60 mm dishes, or 3 × 106/100 mm dishes with Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS). After 40 h of incubation, cultured cardiomyocytes are serum-starved for 8 h before Carbacyclin stimulation. |
Animal experiment: | Mice[3]Ten to twelve week-old male C57BL/6 mice (20-25 g) are used in the experiment. Mice (n = 4) are injected intraperitoneally with 100 μg of Carbacyclin, and are sacrificed at the times indicated. The hearts are excised, and the ventricles are then homogenized with 3 mL of Isogen for the following total RNA extraction procedure[3]. |
References: [1]. Takasuka M, et al. FTIR spectral study of intramolecular hydrogen bonding in thromboxane A2 receptor agonist (U-46619), prostaglandin (PG)E2, PGD2, PGF2 alpha, prostacyclin receptor agonist (carbacyclin), and their related compounds in dilute CCl4 solution: structure-activity relationships. J Med Chem. 1994 Jan 7;37(1):47-56. | |
| Cas No. | 69552-46-1 | SDF | |
| 别名 | Carbaprostacyclin; Carba-PGI2 | ||
| Canonical SMILES | O=C(O)CCC/C=C1C[C@@]2([H])C[C@@H](O)[C@H](/C=C/[C@@H](O)CCCCC)[C@@]2([H])C\1 | ||
| 分子式 | C21H34O4 | 分子量 | 350.49 |
| 溶解度 | DMF: >10 mg/ml (from 6B-PGI2),DMSO: >5 mg/ml (from 6B-PGI2),Ethanol: >20 mg/ml (from 6B-PGI2),PBS pH 7.2: >80 µ g/ml (from 6B-PGI2) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8531 mL | 14.2657 mL | 28.5315 mL |
| 5 mM | 0.5706 mL | 2.8531 mL | 5.7063 mL |
| 10 mM | 0.2853 mL | 1.4266 mL | 2.8531 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet