Carotenoid Mixture
目录号 : GC47044A mixture of carotenoids
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Carotenoid mixture is a mixture of carotenoids that contains the antioxidative and anti-inflammatory carotenoid capsanthin and the antioxidative vitamin A precursor β-carotene , as well as additional carotenoids and carotenoid esters.1,2,3,4
1.Kim, J.S., Lee, W.M., Rhee, H.C., et al.Red paprika (Capsicum annuum L.) and its main carotenoids, capsanthin and β-carotene, prevent hydrogen peroxide-induced inhibition of gap-junction intercellular communicationChem. Biol. Interact.254146-155(2016) 2.Narisawa, T., Fukaura, Y., Hasebe, M., et al.Prevention of N-methylnitrosourea-induced colon carcinogenesis in rats by oxygenated carotenoid capsanthin and capsanthin-rich paprika juiceProc. Soc. Exp. Biol. Med.224(2)116-122(2000) 3.Horie, S., Okuda, C., T., Y., et al.Purified canola lutein selectively inhibits specific isoforms of mammalian DNA polymerases and reduces inflammatory responseLipids45(8)713-721(2010) 4.Negishi, H., Ueda, Y., and Azuma, M.Antioxidant fat-soluble vitamins and lipid peroxides in serumJ. Clin. Biochem. Nutr.26227-234(1999)
| Cas No. | N/A | SDF | |
| Canonical SMILES | CC(/C=C/C1=C(C)C[C@@H](O)CC1(C)C)=C\C=C\C(C)=C\C=C\C=C(C)\C=C\C=C(C)\C=C\C([C@@]2(C)C(C)(C)C[C@H](O)C2)=O | ||
| 分子式 | C40H56O3 (for Capsanthin) | 分子量 | 584.9 |
| 溶解度 | DMF: miscible,DMSO: miscible,Ethanol: miscible,PBS (pH 7.2): soluble | 储存条件 | Store at Room temperature |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7097 mL | 8.5485 mL | 17.0969 mL |
| 5 mM | 0.3419 mL | 1.7097 mL | 3.4194 mL |
| 10 mM | 0.171 mL | 0.8548 mL | 1.7097 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Plasma carotenoid concentrations before and after supplementation with a Carotenoid Mixture
Am J Clin Nutr 1994 Apr;59(4):896-9.PMID:8147336DOI:10.1093/ajcn/59.4.896.
Plasma carotenoid concentrations were determined by HPLC in 11 individuals consuming low-carotenoid diets and after taking a carotenoid supplement. Subjects first consumed low-carotenoid diets for 2 wk, then supplemented these diets daily with 8.5 mg beta-carotene, 3.5 mg alpha-carotene, and 0.5 mg lycopene, from natural sources for 4 wk. Serum cholesterol, triglycerides, and lipoproteins were determined before and after supplementation. After 2 wk on the low-carotenoid diet, plasma concentrations of the three carotenoids fell to approximately 60% of baseline values. One week after supplementation, alpha- and beta-carotene concentrations returned to baseline and by the end of the supplementation period they were significantly higher than baseline values (P < 0.05). Lycopene concentrations increased only slightly. Serum lipids did not change significantly. Overall, plasma concentrations of these carotenoids reflect the amount provided by the supplement. This is the first study reporting increments of serum carotenoids, other than beta-carotene, after supplementation.
Dietary supplementation with a natural Carotenoid Mixture decreases oxidative stress
Eur J Clin Nutr 2003 Sep;57(9):1135-40.PMID:12947433DOI:10.1038/sj.ejcn.1601655.
Objective: To determine whether dietary supplementation with a natural Carotenoid Mixture counteracts the enhancement of oxidative stress induced by consumption of fish oil. Design: A randomised double-blind crossover dietary intervention. Setting: Hugh Sinclair Unit of Human Nutrition, School of Food Biosciences, The University of Reading, Whiteknights PO Box 226, Reading RG6 6AP, UK. Subjects and intervention: A total of 32 free-living healthy nonsmoking volunteers were recruited by posters and e-mails in The University of Reading. One volunteer withdrew during the study. The volunteers consumed a daily supplement comprising capsules containing fish oil (4 x 1 g) or fish oil (4 x 1 g) containing a natural Carotenoid Mixture (4 x 7.6 mg) for 3 weeks in a randomised crossover design separated by a 12 week washout phase. The Carotenoid Mixture provided a daily intake of beta-carotene (6.0 mg), alpha-carotene (1.4 mg), lycopene (4.5 mg), bixin (11.7 mg), lutein (4.4 mg) and paprika carotenoids (2.2 mg). Blood and urine samples were collected on days 0 and 21 of each dietary period. Results: The Carotenoid Mixture reduced the fall in ex vivo oxidative stability of low-density lipoprotein (LDL) induced by the fish oil (P=0.045) and it reduced the extent of DNA damage assessed by the concentration of 8-hydroxy-2'-deoxyguanosine in urine (P=0.005). There was no effect on the oxidative stability of plasma ex vivo assessed by the oxygen radical absorbance capacity test. beta-Carotene, alpha-carotene, lycopene and lutein were increased in the plasma of subjects consuming the Carotenoid Mixture. Plasma triglyceride levels were reduced significantly more than the reduction for the fish oil control (P=0.035), but total cholesterol, HDL and LDL levels were not significantly changed by the consumption of the Carotenoid Mixture. Conclusions: Consumption of the natural Carotenoid Mixture lowered the increase in oxidative stress induced by the fish oil as assessed by ex vivo oxidative stability of LDL and DNA degradation product in urine. The Carotenoid Mixture also enhanced the plasma triglyceride-lowering effect of the fish oil. Sponsorship: : The study was supported by funding from the Greek Studentship Foundation and from Unilever Bestfoods plc. Carotenoids were contributed by Overseal Foods plc.
Distribution of rhodopin and spirilloxanthin between LH1 and LH2 complexes when incorporating Carotenoid Mixture into the membrane of purple sulfur bacterium Allochromatium minutissimum in vitro
Dokl Biochem Biophys 2016 Nov;471(1):383-386.PMID:28058678DOI:10.1134/S1607672916060016.
Carotenoid Mixture enriched by rhodopin and spirilloxanthin was incorporated in LH2 and LH1 complexes from Allochromatium (Alc.) minutissimum in vitro. The maximum incorporating level was ~95%. Rhodopin (56.4%) and spirilloxanthin (13.8%) were incorporated into the LH1 complex, in contrast to the control complex, which contained primarily spirilloxanthin (66.8%). After incorporating, the LH2 complex contained rhodopin (66.7%) and didehydrorhodopin (14.6%), which was close to their content in the control (67.4 and 20.5%, respectively). Thus, it was shown that carotenoids from the total pool are not selectively incorporated into LH2 and LH1 complexes in vitro in the proportion corresponding to the carotenoid content in the complexes in vivo.
Prevention of hepatocellular carcinoma in chronic viral hepatitis patients with cirrhosis by Carotenoid Mixture
Recent Results Cancer Res 2007;174:67-71.PMID:17302186DOI:10.1007/978-3-540-37696-5_6.
Since the incidence of hepatocellular carcinoma in chronic viral hepatitis patients with cirrhosis is very high, it is valuable to develop effective methods for its prevention. In the present study, the effect of a Carotenoid Mixture on hepatocellular carcinoma development was examined. Patients were randomly divided into two groups and treated with a Carotenoid Mixture in addition to conventional antisymptomatic treatment, or antisymptomatic treatment alone. Cumulative incidence of hepatocellular carcinoma development was periodically analyzed using the Kaplan-Meier method. Significantly lower incidence was observed in the carotenoid-treated group compared with the control group in the analysis at year 4.
Commercial Production of Astaxanthin with Paracoccus carotinifaciens
Adv Exp Med Biol 2021;1261:11-20.PMID:33783727DOI:10.1007/978-981-15-7360-6_2.
Paracoccus carotinifaciens is an aerobic Gram-negative bacterium that exhibits motility by a peritrichous flagellum. It produces a Carotenoid Mixture containing astaxanthin as the main component. Selective breeding of P. carotinifaciens has been performed using classical techniques for mutation induction, such as chemical treatment and ultraviolet irradiation, and not using genetic engineering technology. The commercial production of astaxanthin with P. carotinifaciens has been established by optimizing fermentation medium and conditions in the process. Dehydrated P. carotinifaciens is used as a coloring agent for farmed fish and egg yolks. Compared with the administration of chemically synthesized astaxanthin, dehydrated P. carotinifaciens imparts more natural coloration, which is favored by consumers. In addition, astaxanthin-rich carotenoid extracts (ARE) derived from P. carotinifaciens are developed for human nutrition. Animal and clinical studies with ARE for evaluating its efficacy have been conducted and suggested that ARE would be useful for preventing anxiety, stomach ulcer, and retinal damage, as well as improving cognitive function. The efficacy is anticipated to result from not only astaxanthin but also other carotenoids in ARE, such as adonirubin and adonixanthin, in some studies. Hence, astaxanthin commercially produced with P. carotinifaciens has been applied widely in animals and humans.