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Casein Kinase II Inhibitor IV Hydrochloride Sale

目录号 : GC34356

CaseinKinaseIIInhibitorIVHydrochloride是表皮角质形成细胞分化的小分子诱导剂。

Casein Kinase II Inhibitor IV Hydrochloride Chemical Structure

Cas No.:2320347-54-2

规格 价格 库存 购买数量
5mg
¥3,124.00
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10mg
¥4,909.00
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25mg
¥9,818.00
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50mg
¥16,511.00
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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

Kinase experiment:

For reporter gene assays with transiently transfected cells, the cells are typically transfected in 150 mm-diam dishes when 30-40% confluent. A reporter plasmid, pGL3/3.7 kbp-IVLLuc plasmid, is transfected into the human epidermal keratinocytes (NHEKs). After 24 h, the transfected cells are plated into 96-well assay plates and treated with Casein Kinase II Inhibitor IV to a final concentration of 5 μM. After incubation for 2 d, reporter gene activity is measured using the Bright-Glo luciferase assay system[1].

References:

[1]. Hong J, et al. Identification and characterization of small-molecule inducers of epidermal keratinocyte differentiation. ACS Chem Biol. 2007 Mar 20;2(3):171-5.

产品描述

Casein Kinase II Inhibitor IV Hydrochloride is a small-molecule inducer of epidermal keratinocyte differentiation.

Treatment of human epidermal keratinocytes (NHEKs) with Casein Kinase II Inhibitor IV leads to an increase in the early differentiation markers keratins 1 and 10 at 48 h. Increased levels of IVL and TGM are observed in cells treated with Casein Kinase II Inhibitor IV at 72 h and persisted at 96 h. In addition, treated with Casein Kinase II Inhibitor IV expresses loricrin, a terminal differentiation marker, at later time points. Similar results are observed by messenger RNA (mRNA) expression analysis of NHEKs treated with Casein Kinase II Inhibitor IV. At early time points (12 and 24 h), treatment with Casein Kinase II Inhibitor IV leads to the upregulation of keratinocyte early differentiation marker genes, including keratin 1 (5.4-fold) and keratin 10 (5.4-fold). Terminal differentiation marker genes, including IVL (1.8-fold), TGM 1 (4.8-fold), loricrin (3.3-fold), and filaggrin (5.6-fold), are upregulated at late time points (36 and 48 h). These results are consistent with the ability of Casein Kinase II Inhibitor IV to induce differentiation of epidermal progenitor cells into terminally differentiated keratinocytes[1].

[1]. Hong J, et al. Identification and characterization of small-molecule inducers of epidermal keratinocyte differentiation. ACS Chem Biol. 2007 Mar 20;2(3):171-5.

Chemical Properties

Cas No. 2320347-54-2 SDF
Canonical SMILES N#CCCC1=CC=CC(N2C=CC3=CN=C(NC4=CC(OC)=C(OC)C(OC)=C4)N=C32)=C1.Cl
分子式 C24H24ClN5O3 分子量 465.93
溶解度 Soluble in DMSO 储存条件 Store at -20°C
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1 mM 2.1462 mL 10.7312 mL 21.4625 mL
5 mM 0.4292 mL 2.1462 mL 4.2925 mL
10 mM 0.2146 mL 1.0731 mL 2.1462 mL
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