Catechin ((+)-Catechin)
(Synonyms: 儿茶素; (+)-Catechin; Cianidanol; Catechuic acid) 目录号 : GC32841
(+)-Catechin (Cianidanol, Catechinic acid, Catechuic acid) is an antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.
Cas No.:154-23-4
Sample solution is provided at 25 µL, 10mM.
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(+)-Catechin (Cianidanol, Catechinic acid, Catechuic acid) is an antioxidant flavonoid, occurring especially in woody plants as both (+)-catechin and (-)-epicatechin (cis) forms.
The catechin flavonoid reduces proliferation and induces apoptosis of murine lymphoma cells LB02 through modulation of antiapoptotic proteins[1]. Treatment of human prostate cancer cells with catechin induces apoptosis and results in effects on a variety of survival signals suggesting the potential of these compounds as chemopreventive agents for prostate cancer[2]. Catechin is well known to inhibit cyclooxygenase activity with reported IC50 values ranging from 4O μM to 943 μM. It has also been reported to act as an inhibitor of COX-1 and COX-2 with IC50 of about 80 ?M and 130 μM[3].
Dietary catechin significantly delayed tumor onset[5]. Continued intake of catechin, a potential antioxidant, prevents memory regression and DNA oxidative damage in senescence-accelerated (SAMP10) mice. It does not prolong the lifetime of SAMP10 mice, but it does delay brain senescence[6]. (+)-Catechin inhibits intestinal tumor formation and suppresses Focal adhesion kinase activation in the Min/+ Mouse[7].
[1] Daniela Laura Papademetrio, et al. Rev bras farmacogn. 2013, 23(3):455-463. [2] Tayyaba Afsar, et al. Scientific Reports 6. 2016, 23077. [3] Noreen Y, et al. Planta Med. 1998, 64(6):520-4.
Cell experiment: | The Cell viability assay is performed to assess the toxicity of different concentrations of Catechin on MCF-7 cells. Briefly, MCF-7 cells (2×104 cells/well) are plated in 96-well plates and treated with 0 μg/mL Catechin and 160 μg/mL Catechin for 24 hours. Then, 40 μL of the Cell Titer Blue solution is directly added to the wells and incubated at 37°C for 6 hours. The fluorescence is recorded with a 560 nm/590 nm (excitation/emission) filter set using a microplate fluorescence reader, and the IC50 is calculated. Quadruplet samples are run for each concentration of Catechin in three independent experiments[2]. |
Animal experiment: | Rats[3]Twelve weeks old, healthy male rats weighing 200 to 230 g are used in this study. Rats are divided into four experimental groups (n=9 each) for one vehicle and three groups of Catechin (three doses). The doses of Catechin are prepared at 50, 100, 200 mg/kg in 0.25% w/v sodium carboxy methylcellulose (CMC) and administered orally for 7 days prior to and during the experimental trials. Episodic memory, the conscious memory of the past experiences is evaluated in this study[3]. |
References: [1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9. | |
| Cas No. | 154-23-4 | SDF | |
| 别名 | 儿茶素; (+)-Catechin; Cianidanol; Catechuic acid | ||
| Canonical SMILES | O[C@@H]1[C@@H](C2=CC(O)=C(O)C=C2)OC3=CC(O)=CC(O)=C3C1 | ||
| 分子式 | C15H14O6 | 分子量 | 290.27 |
| 溶解度 | DMSO : ≥ 100 mg/mL (344.51 mM) | 储存条件 | 4°C, away from moisture and light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4451 mL | 17.2253 mL | 34.4507 mL |
| 5 mM | 0.689 mL | 3.4451 mL | 6.8901 mL |
| 10 mM | 0.3445 mL | 1.7225 mL | 3.4451 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet