(-)-Catechin gallate ((-)-Catechin 3-gallate)
(Synonyms: 儿茶素没食子酸酯; (-)-Catechin 3-gallate; (-)-Catechin 3-O-gallate) 目录号 : GC32957
A polyphenol with diverse biological activities
Cas No.:130405-40-2
Sample solution is provided at 25 µL, 10mM.
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(–)-Catechin gallate ((–)-CG) is a catechin polyphenol that has been found in green tea extracts with diverse biological activities.1,2,3,4 It inhibits proliferation of MDA-MB-231 breast cancer cells and reduces VEGF-induced tyrosine phosphorylation of VEGF receptor 2 (VEGFR2) in bovine aortic endothelial cells (BAECs) in a concentration-dependent manner.1,2 (–)-CG is a competitive inhibitor of the facilitative glucose transporter GLUT4 (Ki = 90 μM) and inhibits methylglucose uptake by isolated rat adipocytes (IC50 = 50 μM).3 It is also lytic against T. cruzi amastigotes, with a 50% bactericidal concentration (MBC50) value of 48 pM.4
1.Lamy, S., Gingras, D., and Béliveau, R.Green tea catechins inhibit vascular endothelial growth factor receptor phosphorylationCancer Res.62(2)381-385(2002) 2.Sartippour, M.R., Heber, D., Ma, J., et al.Green tea and its catechins inhibit breast cancer xenograftsNutr. Cancer40(2)149-156(2001) 3.Strobel, P., Allard, C., Perez-Acle, T., et al.Myricetin, quercetin and catechin-gallate inhibit glucose uptake in isolated rat adipocytesBiochem. J.386(Pt 3)471-478(2005) 4.Paveto, C., Güida, M.C., Esteva, M.I., et al.Anti-Trypanosoma cruzi activity of green tea (Camellia sinensis) catechinsAntimicrob. Agents Chemother.48(1)69-74(2004)
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Cell experiment: |
Human tongue squamous carcinoma (CAL27) cells and human salivary gland carcinoma (HSG) cells are used. Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 mL of the growth medium containing 2×104 cells/well for a 1-day exposure, 1.5×104 cells/ well for a 2-day exposure and 1×104 cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to (-)-Catechin gallate (100, 200, 300, 400, and 500 μM) and 100 Units/mL catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay, which is based on the uptake and accumulation of the supravital dye, neutral red (NR)[1]. |
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References: [1]. Babich H, et al. In vitro cytotoxicity of (-)-catechin gallate, a minor polyphenol in green tea. Toxicol Lett. 2007 Jul 10;171(3):171-80. |
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| Cas No. | 130405-40-2 | SDF | |
| 别名 | 儿茶素没食子酸酯; (-)-Catechin 3-gallate; (-)-Catechin 3-O-gallate | ||
| Canonical SMILES | O=C(O[C@H]1[C@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4 | ||
| 分子式 | C22H18O10 | 分子量 | 442.37 |
| 溶解度 | DMSO : 100 mg/mL (226.06 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2606 mL | 11.3028 mL | 22.6055 mL |
| 5 mM | 0.4521 mL | 2.2606 mL | 4.5211 mL |
| 10 mM | 0.2261 mL | 1.1303 mL | 2.2606 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet