CAY10732
目录号 : GC43206CAY10732是一种检测一氧化碳的荧光探针。
Cas No.:855751-82-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Protocol for Imaging of CO in Living Cells[1]： |
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Stock Solution Preparation：Stock solutions of CAY10732 (2 mM), PdCl2 (2 mM), and CORM-3 (10 mM) were separately prepared in HPLC grade DMSO and used freshly. CAY10732 (1 μM) and PdCl2 (1 μM) in PBS buffer (10 mM, pH 7.4, with 0.5% DMSO, v/v) was prepared in use. 1.HeLa cells were seeded in a 24-well culture plate for one night before cell imaging experiments. 2.In a typical experiment of cell imaging, as controls, living cells were incubated with CAY10732 (1 μM) and a mixture of CAY10732 and PdCl2 (1 μM each) at 37℃ for 30 min, respectively, and they were imaged after washing with PBS for three times. 3.For imaging of exogenous CO, HeLa cells were pretreated with CORM-3 (1, 5, and 10 μM, respectively) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl2 (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged. 4.For imaging of heme stimulation produced CO, HeLa cells were pretreated with heme (100 μM) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl2 (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged. This protocol only provides a guideline, and should be modified according to your specific needs. |
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References: [1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017). |
CAY10732 functions as a fluorescent agent designed to identify the presence of carbon monoxide.1 When exposed to palladium (Pd) and carbon monoxide, CAY10732 initiates the Pd0-mediated Tsuji-Trost reaction, resulting in the liberation of 2,7-dichlorofluorescein. The fluorescence emitted by this compound serves as an indicator of carbon monoxide production, both within controlled laboratory settings and within living cells. The excitation/emission peaks of 2,7-dichlorofluorescein are measured at 493/527 nm, respectively[1].
References:
[1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017).
CAY10732是一种检测一氧化碳的荧光探针。在钯(Pd)和一氧化碳存在下，CAY10732通过pd0介导的Tsuji-Trost反应释放2,7-二氯荧光素，其荧光可用于体外和活细胞中检测一氧化碳的产生。2,7-二氯荧光素的激发/发射最大值分别为493/527 nm[1]。
| Cas No. | 855751-82-5 | SDF | |
| 化学名 | 3',6'-bis(2-propen-1-yloxy)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one | ||
| Canonical SMILES | O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OCC=C)C=C4)OC5=C3C=CC(OCC=C)=C5 | ||
| 分子式 | C26H20O5 | 分子量 | 412.4 |
| 溶解度 | DMF: 12 mg/ml,DMSO: 10 mg/ml,Ethanol: 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4248 mL | 12.1242 mL | 24.2483 mL |
| 5 mM | 0.485 mL | 2.4248 mL | 4.8497 mL |
| 10 mM | 0.2425 mL | 1.2124 mL | 2.4248 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。