Cecropin A
(Synonyms: 抗菌肽A) 目录号 : GC31773
An antimicrobial peptide
Cas No.:80451-04-3
Sample solution is provided at 25 µL, 10mM.
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Cecropin A is an antimicrobial peptide that has been found in H. cecropia.1,2 It induces apoptosis in C. albicans when used at a concentration of 5 ?M.2 Cecropin A is active against the planktonic and uropathogenic E. coli strain CFT073 (MIC = 100 ?g/ml) and increases survival of CFT073-infected G. mellonella larvae.1 Cecropin A (15 mg/kg) decreases enrichment of the gut microflora Bacteroidaceae and Enterobacteriaceae and increases gut levels of Lactobacillus, as well as increases survival and decreases body weight loss in a mouse model of inflammatory bowel disease (IBD) induced by dextran sulfate .3
1.Kalsy, M., Tonk, M., Hardt, M., et al.The insect antimicrobial peptide cecropin A disrupts uropathogenic Escherichia coli biofilmsNPJ Biofilms Microbiomes6(1)6(2020) 2.Yun, J., and Lee, D.G.Cecropin A-induced apoptosis is regulated by ion balance and glutathione antioxidant system in Candida albicansIUBMB Life68(8)652-662(2016) 3.Zhai, Z., Zhang, F., Cao, R., et al.Cecropin A alleviates inflammation through modulating the gut microbiota of C57BL/6 mice with DSS-induced IBDFront. Microbiol.101595(2019)
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Cell experiment: |
Briefly, 5 × 105 cells/mL in RPMI 1640 supplemented with 10% heat-inactivated FCS are placed onto 96-well plates. Cecropin A is added to cell cultures at a final concentration of 10, 20, 30, 40 and 50 μM and cells are incubated for 24 h at 37°C in a humidified atmosphere with 5% CO2. Then, 20 μL MTT (0.5 mg/mL) is added to each well and the plate is incubated for 4 h at 37°C. The MTT solution is removed and isopropyl alcohol containing 0.04 N hydrochloric acid is added to each well to dissolve the formazan crystal. Absorbance is determined on a spectrophotometric microplate reader at a test wavelength of 550 nm and a reference wavelength of 620 nm. The absorbance of the cells incubated in the absence of cecropin A (untreated cells) is set at 100%. Results are expressed as percentage of cell viability[1]. |
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References: [1]. Cerón JM, et al. The antimicrobial peptide cecropin A induces caspase-independent cell death in human promyelocytic leukemia cells. Peptides. 2010 Aug;31(8):1494-503. |
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| Cas No. | 80451-04-3 | SDF | |
| 别名 | 抗菌肽A | ||
| Canonical SMILES | Lys-Trp-Lys-Leu-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Gln-Asn-Ile-Arg-Asp-Gly-Ile-Ile-Lys-Ala-Gly-Pro-Ala-Val-Ala-Val-Val-Gly-Gln-Ala-Thr-Gln-Ile-Ala-Lys-NH2 | ||
| 分子式 | C184H313N53O46 | 分子量 | 4003.78 |
| 溶解度 | Water : ≥ 50 mg/mL (12.14 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.2498 mL | 1.2488 mL | 2.4976 mL |
| 5 mM | 0.05 mL | 0.2498 mL | 0.4995 mL |
| 10 mM | 0.025 mL | 0.1249 mL | 0.2498 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet