Cecropin B
(Synonyms: 天蚕丝抗菌肽) 目录号 : GC32991
An antimicrobial peptide
Cas No.:80451-05-4
Sample solution is provided at 25 µL, 10mM.
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Cecropin B is an antimicrobial peptide.1 It is active against the Gram-positive bacteria S. aureus, B. subtilis, Sporosarcina, and Curtobacterium (MICs = 0.89, 0.98, 0.88, and 0.97 ?M, respectively) and the Gram-negative bacteria E. coli, S. pullorum, and P. aeruginosa (MICs = 0.5, 0.78, and 0.98, respectively). Cecropin B also induces membrane permeabilization in, and is active against the plant pathogenic fungi R. solani (MIC = 9.8 ?M).2
1.Wang, X., Zhu, M., Yang, G., et al.Expression of cecropin B in Pichia pastoris and its bioactivity in vitroExp. Ther. Med.2(4)655-660(2011) 2.Oard, S., Rush, M.C., and Oard, J.H.Characterization of antimicrobial peptides against a US strain of the rice pathogen Rhizoctonia solaniJ. Appl. Microbiol.97(1)169-180(2004)
Animal experiment: | Mice[1]Thirty ICR mice are enrolled in the study, and the Pseudomonas aeruginosa infection model is reproduced by excision of the full layer of dorsal skin with an area of 1 cm x 1 cm. Then they are randomly divided into C (control, n=10, with wet compress of isotonic saline at 3 postinjury hour (PIH)) , M (with hydropathic compress of 100 g/L mafenide at 3 PIH), A (with wet compress of 1 000 mg/L Cecropin B at 3 PIH) groups. The changes in body temperature and hemogram in each group are determined before and 4 days after injury[2]. |
References: [1]. Zhou X et al. Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway. Sci Rep. 2016 Jun 14 | |
| Cas No. | 80451-05-4 | SDF | |
| 别名 | 天蚕丝抗菌肽 | ||
| Canonical SMILES | Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Met-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu-NH2 | ||
| 分子式 | C176H302N52O41S | 分子量 | 3834.67 |
| 溶解度 | Water : 20 mg/mL (5.22 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.2608 mL | 1.3039 mL | 2.6078 mL |
| 5 mM | 0.0522 mL | 0.2608 mL | 0.5216 mL |
| 10 mM | 0.0261 mL | 0.1304 mL | 0.2608 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet