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Certolizumab pegol Sale

(Synonyms: 赛妥珠单抗,Certolizumab; CDP870) 目录号 : GC65487

Certolizumab pegol(Certolizumab)是一种重组、聚乙烯糖基化、抗原结合片段的人源化单克隆抗体,选择性靶向和中和肿瘤坏死因子-α(TNF-α)。Certolizumab pegol可用于类风湿关节炎和克罗恩病研究。

Certolizumab pegol Chemical Structure

Cas No.:428863-50-7

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1mg
¥1,620.00
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5mg
¥3,420.00
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10mg
¥5,400.00
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Sample solution is provided at 25 µL, 10mM.

Description

Certolizumab pegol (Certolizumab) is a recombinant, polyethylene glycolylated, antigen-binding fragment of a humanized monoclonal antibody that selectively targets and neutralizes tumour necrosis factor-α (TNF-α). Certolizumab pegol can be used for rheumatoid arthritis and Crohn disease research[1-2].

Certolizumab pegol neutralizes soluble TNF-α with an IC90 of 3ng/mL. Preincubation of human monocytes with certolizumab pegol at concentrations of 1μg/mL and above completely inhibited lipopolysaccharide(LPS)-induced IL-1β production, and certolizumab pegol did not induce apoptosis of activated human monocytes or peripheral blood lymphocytes (PBLs), nor did it cause changes in the integrity of polymorphonuclear granules (PMNs)[2]. In addition, certolizumab pegol can directly induce non-apoptotic cell death in transmembrane TNF-α-expressing cells[4].

Certolizumab pegol (10μg, single dose) reduced the severity of acute pancreatitis, and serum amylase, lipase, and lactate dehydrogenase levels were significantly reduced in the certolizumab pegol-treated group[3]. Compared with the control group (non-inflamed tissue), certolizumab pegol (2mg/kg) can effectively penetrate into the inflamed arthritic paws of mice and improve arthritis in mice[5].

References:
[1] Deeks ED. Certolizumab pegol: a review of its use in the management of rheumatoid arthritis. Drugs. 2013 Jan;73(1):75-97.
[2] Nesbitt A, et al. Mechanism of action of certolizumab pegol (CDP870): in vitro comparison with other anti-tumor necrosis factor alpha agents. Inflamm Bowel Dis. 2007 Nov;13(11):1323-32.
[3] Kosekli M A, Sungurtekin U, Cobankara V, et al. Effects of certolizumab on cerulein-induced acute pancreatitis in rats[J]. Pancreas, 2016, 45(8): 1120-1125.
[4] Ueda N, Tsukamoto H, Mitoma H, et al. The cytotoxic effects of certolizumab pegol and golimumab mediated by transmembrane tumor necrosis factor α[J]. Inflammatory bowel diseases, 2013, 19(6): 1224-1231.
[5] Palframan R, Airey M, Moore A, et al. Use of biofluorescence imaging to compare the distribution of certolizumab pegol, adalimumab, and infliximab in the inflamed paws of mice with collagen-induced arthritis[J]. Journal of immunological methods, 2009, 348(1-2): 36-41.

Certolizumab pegol(Certolizumab)是一种重组、聚乙烯糖基化、抗原结合片段的人源化单克隆抗体,选择性靶向和中和肿瘤坏死因子-α(TNF-α)。Certolizumab pegol可用于类风湿关节炎和克罗恩病研究[1-2]

Certolizumab pegol可中和可溶性TNF-α,IC90为3ng/mL。人单核细胞与浓度为1μg/mL及以上浓度的Certolizumab pegol预孵育完全抑制了脂多糖(LPS)诱导的IL-1β的产生,并且Certolizumab pegol不会诱导活化的人单核细胞或外周血淋巴细胞(PBL)凋亡,也不会导致多形核颗粒(PMN)完整性发生变化[2]。此外,Certolizumab pegol可以直接诱导跨膜TNF-α表达细胞中的非凋亡细胞死亡[4]

Certolizumab pegol(10μg,单剂量)可降低急性胰腺炎的严重程度,并且Certolizumab pegol治疗组血清淀粉酶、脂肪酶和乳酸脱氢酶水平显著降低[3]。与对照组相比(非炎症组织),Certolizumab pegol(2mg/kg)能有效地渗透小鼠发炎的关节炎爪子,改善小鼠关节炎[5]

实验参考方法

Cell experiment [1]:

Cell lines

Human dermal microvascular endothelial cells (HMVECs)

Preparation Method

HMVECs was stimulated with TNF-α (25ng/ml) for 24 hours in the presence or absence of varying concentrations of certolizumab pegol or Ms-Ig. The cell culture supernatant was collected and the level of HMVEC chemokines was detected by elisa.

Reaction Conditions

0-10μg/mL, 24h

Applications

Certolizumab pegol inhibits TNF-α-induced increases in angiogenic chemokines in a dose-dependent manner.

Animal experiment [2]:

Animal models

Rat model of acute edematous pancreatitis

Preparation Method

Healthy Wistar Albino male rats (n = 36) were randomly divided into 4 groups (9 rats in each group): group 1, control group; group 2, Certolizumab pegol group; group 3, cerulein group; and group 4, cerulein + Certolizumab pegol group.

Dosage form

10μg, single dose, i.p.

Applications

Certolizumab pegol reduced the severity of acute pancreatitis, and serum amylase, lipase and lactate dehydrogenase levels were significantly reduced in the certolizumab pegol-treated group.

References:
[1] Shu Q, Amin MA, Ruth JH, Campbell PL, Koch AE. Suppression of endothelial cell activity by inhibition of TNFα. Arthritis Res Ther. 2012 Apr 25;14(2):R88.
[2] Kosekli M A, Sungurtekin U, Cobankara V, et al. Effects of certolizumab on cerulein-induced acute pancreatitis in rats[J]. Pancreas, 2016, 45(8): 1120-1125.

化学性质

Cas No. 428863-50-7 SDF Download SDF
别名 赛妥珠单抗,Certolizumab; CDP870
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