Chlorogenic acid
(Synonyms: 绿原酸; 3-O-Caffeoylquinic acid; Heriguard; NSC-407296) 目录号 : GN10308
Chlorogenic acid是一种有效的神经保护剂，同时具有抗病毒、抗真菌、抗氧化和抗肿瘤作用。Chlorogenic acid还参与调节葡萄糖和脂质代谢，改善胰岛素敏感性。
Cas No.:327-97-9
Sample solution is provided at 25 µL, 10mM.
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Chlorogenic acid is a potent neuroprotective agent and also has antiviral,antifungal antioxidant and antitumor properties. CGA is also involved in regulating glucose and lipid metabolism and improving insulin sensitivity [1].
Chlorogenic acid (25, 50 μM; 24h) dose-dependently reduced the expression of p-c-Myc and increased the expression of p21 in U87MG and M059J cells[1]; Chlorogenic acid (30, 60 μM, 24h) inhibited the proliferation of B16 melanoma cells; After co-incubation of B16 melanoma cells with 8-methoxypsoralen (8-MOP), chlorogenic acid (500 μM, 24h) reduced melanin production in B16 melanoma cells and inhibited tyrosinase activity[2].
Chlorogenic acid (75mg/kg, ip, bid) inhibited the tumor growth of rat C6 glioma, with a tumor volume inhibition rate of 57%[1]. In the LPS-induced acute lung injury model, chlorogenic acid (50 mg/kg, ip) inhibited the LPS-induced increase in lung tissue MPO activity and the migration of neutrophils (PMN) to bronchoalveolar lavage fluid (BALF), and blocked the release of nitric oxide (NO) induced by LPS stimulation [3]. Chlorogenic acid (100 mg/kg, ip, biw, 6 weeks) treatment inhibited HFD-induced liver steatosis and insulin resistance. Chlorogenic acid inhibited the expression of liver Pparγ, Cd36, Fabp4 and Mgat1 genes, reduced inflammation in the liver and white adipose tissue, and was accompanied by a decrease in the mRNA levels of macrophage marker genes (including F4/80, Cd68, Cd11b, Cd11c, and Tnfα, Mcp-1 and Ccr2 encoding inflammatory proteins) [4].
References:
[1]. Huang S, Wang LL, Xue NN, Li C, Guo HH, Ren TK, Zhan Y, Li WB, Zhang J, Chen XG, Han YX, Zhang JL, Jiang JD. Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation. Theranostics. 2019 Sep 19;9(23):6745-6763.
[2]. Li HR, Habasi M, Xie LZ, Aisa HA. Effect of chlorogenic acid on melanogenesis of B16 melanoma cells. Molecules. 2014 Aug 25;19(9):12940-8.
[3]. Zhang X, Huang H, Yang T, Ye Y, Shan J, Yin Z, Luo L. Chlorogenic acid protects mice against lipopolysaccharide-induced acute lung injury. Injury. 2010 Jul;41(7):746-52.
[4]. Ma Y, Gao M, Liu D. Chlorogenic acid improves high fat diet-induced hepatic steatosis and insulin resistance in mice. Pharm Res. 2015 Apr;32(4):1200-9.
Chlorogenic acid是一种有效的神经保护剂，同时具有抗病毒、抗真菌、抗氧化和抗肿瘤作用。Chlorogenic acid还参与调节葡萄糖和脂质代谢，改善胰岛素敏感性[1]。
Chlorogenic acid（25,50 μM ;24h）剂量依赖性地降低了U87MG和M059J细胞中p-c-Myc的表达并增加了p21的表达[1]; Chlorogenic acid（30,60 µM，24h）抑制了B16黑色素瘤细胞的增殖; Chlorogenic acid（500 μM，24h）在B16黑色素瘤细胞和8-甲氧基补骨脂素（8-MOP）共孵育后，B16黑色素瘤细胞中黑色素生成减少，同时抑制酪氨酸酶活性[2]。
Chlorogenic acid (75mg/kg，ip，bid)抑制大鼠C6胶质瘤的肿瘤生长，其肿瘤体积抑制率为57%[1]。在LPS诱发的急性肺损伤模型中，Chlorogenic acid (50mg/kg，ip)抑制了LPS诱导的肺组织MPO活性升高和中性粒细胞(PMN)向支气管肺泡灌洗液(BALF)的迁移,阻止LPS刺激引起的一氧化氮(NO)的释放[3]。Chlorogenic acid (100mg/kg，ip，biw，6周)治疗可抑制HFD诱导的肝脏脂肪变性和胰岛素抵抗，绿原酸抑制肝脏Pparγ、Cd36、Fabp4和Mgat1基因的表达，减少肝脏和白色脂肪组织的炎症，并伴随巨噬细胞标志基因（包括F4/80、Cd68、Cd11b、Cd11c，以及编码炎症蛋白的Tnfα、Mcp-1和Ccr2）的mRNA水平降低[4]。
| Cell experiment [1]: | |
Cell lines | U87MG and M059J cells |
Preparation Method | Chlorogenic acid ( 25 µM, 50 µM ) were treated with U87MG and M059J cells for 24 hours. |
Reaction Conditions | Chlorogenic acid (25,50) μM ;24h |
Applications | Chlorogenic acid dose-dependently decreased p-c-Myc expression and increased p21 expression in cell lines confirming the differentiation-inducing effect of Chlorogenic acid in solid tumors. |
| Animal experiment [1]: | |
Animal models | Xenograft tumor model |
Preparation Method | The animals received an intraperitoneal (ip) injection of Chlorogenic acid (directly dissolved in saline; 25, or 50, or 200 mg/kg/d) for 30 days, and the same volume of saline was used as a control. |
Dosage form | Chlorogenic acid ( 25,50 and 200mg/kg, ip),30 days |
Applications | Chlorogenic acid treatment with ip administration of 25 mg/kg/d, 50 mg/kg/d, or 200 mg/kg/d slowed down the growth of the tumor mass, yielding 44.0%, 80.1% or 83.1% tumor volume inhibition of Huh7 tumors and 39.9%, 84.6%, or 86.3% tumor reduction of H446 tumors, respectively |
References: [1]. Huang S, Wang LL, Xue NN, Li C, Guo HH, Ren TK, Zhan Y, Li WB, Zhang J, Chen XG, Han YX, Zhang JL, Jiang JD. Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation. Theranostics. 2019 Sep 19;9(23):6745-6763. | |
| Cas No. | 327-97-9 | SDF | |
| 别名 | 绿原酸; 3-O-Caffeoylquinic acid; Heriguard; NSC-407296 | ||
| 化学名 | (1S,3R,4R,5R)-3-[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxy-1,4,5-trihydroxycyclohexane-1-carboxylic acid | ||
| Canonical SMILES | C1C(C(C(CC1(C(=O)O)O)OC(=O)C=CC2=CC(=C(C=C2)O)O)O)O | ||
| 分子式 | C16H18O9 | 分子量 | 354.31 |
| 溶解度 | ≥ 13.8mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8224 mL | 14.1119 mL | 28.2239 mL |
| 5 mM | 0.5645 mL | 2.8224 mL | 5.6448 mL |
| 10 mM | 0.2822 mL | 1.4112 mL | 2.8224 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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