Chlorpyrifos-methyl
(Synonyms: 甲基毒死蜱) 目录号 : GC47084
An organophosphate insecticide
Cas No.:5598-13-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Chlorpyrifos-methyl is an organophosphate insecticide that induces 100, 100, 63, and 97.3% mortality of the adult stored-grain pests L. bostrychophila, L. decolor, L. entomophila, and L. paeta, respectively, when applied to grain at a dose of 10 mg/kg.1 It also reduces the number of live progeny produced by L. bostrychophila, L. decolor, L. entomophila, and L. paeta by 100, 100, 30.4, and 41.1%, respectively, when applied to grain at a dose of 2.5 mg/kg. Chlorpyrifos-methyl (3 μM) reduces outgrowth of axon-like processes in mouse N2a neuroblastoma cells.2 Administration of chlorpyrifos-methyl (4-100 mg/kg) to pregnant female mice decreases H19 gene methylation in sperm as well as decreases anogenital distance and increases thymus and epididymis weights and serum testosterone levels in male offspring.3 Formulations containing chlorpyrifos-methyl have been used to control stored-grain pest and mosquito populations.
1.Nayak, M.K., and Daglish, G.J.Combined treatments of spinosad and chlorpyrifos-methyl for management of resistant psocid pests (Psocoptera: Liposcelididae) of stored grainPest Manag. Sci.63(1)104-109(2007) 2.Sachana, M., Flaskos, J., and Hargreaves, A.J.Effects of chlorpyrifos and chlorpyrifos-methyl on the outgrowth of axon-like processes, tubulin, and GAP-43 in N2a CellsToxicol. Mech. Methods15(6)405-410(2005) 3.Shin, H.S., Seo, J.H., Jeong, S.H., et al.Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos-methyl exposure during organogenesis periodEnviron. Toxicol.30(12)1355-1363(2015)
| Cas No. | 5598-13-0 | SDF | |
| 别名 | 甲基毒死蜱 | ||
| Canonical SMILES | ClC1=NC(OP(OC)(OC)=S)=C(Cl)C=C1Cl | ||
| 分子式 | C7H7Cl3NO3PS | 分子量 | 322.5 |
| 溶解度 | Chloroform: Slightly Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1008 mL | 15.5039 mL | 31.0078 mL |
| 5 mM | 0.6202 mL | 3.1008 mL | 6.2016 mL |
| 10 mM | 0.3101 mL | 1.5504 mL | 3.1008 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Dietary Chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua)
Fish Shellfish Immunol 2022 Aug;127:549-560.PMID:35803506DOI:10.1016/j.fsi.2022.06.060.
Inclusion of new environmental toxicants increase with the amount of plant ingredients substituting marine proteins and oils in feed for farmed Atlantic salmon (Salma salar). Agricultural pesticides like Chlorpyrifos-methyl, present in commercial salmon feeds, may affect salmon immune and detoxification responses. Atlantic cod (Gadus morhua), surrounding the net pens, grazing on feces and uneaten pellets may be affected accordingly. The aim of this study was to analyze transcription responses in Atlantic cod head kidney tissue and isolated leukocytes following dietary Chlorpyrifos-methyl inclusions and possible interactions with proinflammatory signals. Head kidney tissues and leukocytes were isolated from cod fed diets contaminated with Chlorpyrifos-methyl (0.5 mg/kg, 2.4 mg/kg, 23.2 mg/kg) for 30 days. The isolated leukocytes were further challenged with bacteria (lipopolysaccharide (LPS), virus (polyinosinic acid:polycytidylic acid (PIC) mimic and l-arginine, an immuno-modulating amino acid, in vitro. The LPS-induced transcription of the interleukin genes il-1β, il-6, il-8 increased in leukocytes isolated from cod fed Chlorpyrifos-methyl 23.2 mg/kg, compared to cod fed the control diet, indicating increased inflammation. Transcriptional levels of carnitine palmitoyl transferase (cpt1a), aryl hydrogen receptor (ahr) and catalase (cat) were all reduced by dietary inclusions of Chlorpyrifos-methyl in the leukocytes. The findings suggests that dietary Chlorpyrifos-methyl exposure impair inflammation, detoxification and redox signaling in cod leukocytes.
[Chlorpyrifos-methyl, Pirimiphos-Methyl and Fenitrothion Residues in Commercial Wheat Products]
Shokuhin Eiseigaku Zasshi 2018;59(5):228-233.PMID:30429422DOI:10.3358/shokueishi.59.228.
We measured the concentrations of organophosphorus pesticide （Chlorpyrifos-methyl, pirimiphos-methyl and fenitrothion） residues in domestic and imported commercial wheat products （flour, noodles, cookies） collected from 2008 to 2016. Chlorpyrifos-methyl and fenitrothion in domestic flour samples were detected in 16 out of 34 samples at levels of ＜0.001 to 0.016 ppm, and in 14 out of 34 samples at levels of ＜0.001 to 0.004 ppm. Chlorpyrifos-methyl was detected in 22 out of 38 domestic cookies at levels of ＜0.001 to 0.054 ppm （median: 0.001 ppm）. Relatively high concentrations of Chlorpyrifos-methyl （0.005 to 0.054 ppm） were found in six domestic cookies containing wheat bran. Pirimiphos-methyl was detected in 32 out of 68 cookies from foreign countries at levels of ＜0.001 to 0.11 ppm. Pirimiphos-methyl was detected frequently in products imported from Europe.
Terahertz sensing of Chlorpyrifos-methyl using metamaterials
Food Chem 2017 Mar 1;218:330-334.PMID:27719917DOI:10.1016/j.foodchem.2016.09.032.
By squeezing electromagnetic energy into small volumes near a metal-dielectric interface, plasmonics provide many routes to enhance and manipulate light-matter interactions, which presents new strategies for signal enhancing technologies. As an extension of the ideas of plasmonics to the terahertz (THz) range, metamaterials have shown great potential in sensing applications. In this study, terahertz time-domain spectroscopy (THz-TDS) combined with metamaterials was used to detect Chlorpyrifos-methyl (CM), which is one type of the broad-spectrum organophosphorus pesticides. The results demonstrate that sensitivity is greatly improved using THz metamaterials, with the limit of detection (LOD) of CM reaching 0.204mgL-1, which is lower than the World Health Organization's provisional guideline limit for CM in vegetables (1mgL-1). The results indicated that THz spectroscopy combined with metamaterials could be a valuable method for highly sensitive THz applications, presenting a new strategy for food quality and safety control in the future.
The Simultaneous Determination of Chlorpyrifos-Ethyl and -Methyl with a New Format of Fluorescence-Based Immunochromatographic Assay
Biosensors (Basel) 2022 Nov 11;12(11):1006.PMID:36421124DOI:10.3390/bios12111006.
The improper and excessive use in agriculture of Chlorpyrifos-methyl (CPSM) and chlorpyrifos-ethyl (CPSE) may affect the health of human beings. Herein, a fluorescence-based immunochromatographic assay (FICA) was developed for the simultaneous determination of CPSM and CPSE. A monoclonal antibody (mAb) with equal recognition of CPSM and CPSE was generated by the careful designing of haptens and screening of hybridoma cells. Instead of labeling fluorescence with mAb, the probe was labeled with goat-anti-mouse IgG (GAM-IgG) and pre-incubated with mAb in the sample. The complex could compete with CPS by coating antigen in the test line. The new format of FICA used goat-anti-rabbit IgG (GAR-IgG) conjugated with rabbit IgG labeled with fluorescence microspheres as an independent quality control line (C line). The novel strategy significantly reduced nonspecific reactions and increased assay sensitivity. Under the optimal conditions, the proposed FICA showed a linear range of 0.015-64 mg/L and limit of detection (LOD) of 0.015 mg/L for both CPSE and CPSM. The average recoveries of CPS from spiked food samples by FICA were 82.0-110.0%. The accuracy was similar to the gas chromatography-tandem mass spectrometry (GC-MS/MS) results. The developed FICA was an ideal on-site tool for rapid screening of CPS residues in foods.
Computational insights into the reactivity of chlorpyrifos and Chlorpyrifos-methyl toward singlet oxygen
J Mol Model 2021 Sep 10;27(10):282.PMID:34505937DOI:10.1007/s00894-021-04897-9.
A complete mechanism for the •OH-initiated atmospheric decomposition of the pesticides chlorpyrifos and Chlorpyrifos-methyl is proposed, incorporating additional studies on the competing reaction with singlet oxygen. The computational study is based on density functional theory (DFT) at the double-hybrid functional level to treat static correlation in the calculations of energy barriers. Reaction of the P-bonded intermediate with 1O2 has a small energy barrier of ~ 2 kcal mol-1, generating the Oxone compound and the HOSO• radical, with a reaction free energy of - 49.8 kcal/mol for the chlorpyrifos reaction pathway. Direct reaction of the pesticides with singlet oxygen is unlikely to happen due to the exceedingly high energy barrier of ~ 52 kcal/mol. However, in aqueous solution, the activation energy reduces dramatically and changes the reaction thermodynamics, making it kinetically accessible and thermodynamically viable.