Cholesterol-d7
(Synonyms: 胆固醇 d7) 目录号 : GC47085
An internal standard for the quantification of cholesterol
Cas No.:83199-47-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cholesterol-d7 is intended for use as an internal standard for the quantification of cholesterol by GC- or LC-MS. Cholesterol is a major sterol produced in mammalian cells that is required for cell viability and proliferation.1 It is a component of mammalian cell membranes that interacts with membrane phospholipids, sphingolipids, and proteins to influence their behavior. Cholesterol is a precursor of steroid hormones, bile acids, and the active form of vitamin D. Impaired cholesterol homeostasis is related to development of various diseases including fatty liver, diabetes, gallstones, dyslipidemia, atherosclerosis, heart attack, and stroke.2
1.Ohvo-RekilÄ, H., Ramstedt, B., LeppimÄki, P., et al.Cholesterol interactions with phospholipids in membranesProg. Lipid Res.41(1)66-97(2002) 2.Yamanashi, Y., Takada, T., and Suzuki, H.Associations between lifestyle-related diseases and transporters involved in intestinal absorption and biliary excretion of cholesterolBiol. Pharm. Bull.41(1)1-10(2018)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.54 mL | 12.7 mL | 25.4001 mL |
| 5 mM | 0.508 mL | 2.54 mL | 5.08 mL |
| 10 mM | 0.254 mL | 1.27 mL | 2.54 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。