Cholesterol
(Synonyms: 胆固醇) 目录号 : GC17398
A sterol
Cas No.:57-88-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cholesterol is the major sterol in mammals, and its importance in fundamental cellular processes is becoming more appreciated. IC50 value:Target:In vitro: GT1-7 hypothalamic cells subjected to cholesterol depletion in vitro produced 20-31% reductions in cellular cholesterol content, similar to the decrease in cholesterol synthesis observed in diabetes [1].In vivo:
胆固醇是哺乳动物体内的主要甾醇，在基本细胞过程中的重要性越来越受到重视。IC50值：目标：In vitro：在体外，经胆固醇耗竭的GT1-7下丘脑细胞产生了20-31%的细胞胆固醇含量减少，类似于糖尿病中观察到的胆固醇合成减少 [1]。In vivo: 暂无资料报道。
References:
[1]. Laverdy OG, et al. Effects of glycemic control upon serum lipids and lipid transfers to HDL in patients with type 2 diabetes mellitus: novel findings in unesterified cholesterol status. Experimental and Clinical Endocrinology & Diabetes, 2015, 123(4):232-239
[2]. Sandy Huey-Jen Hsu, et al. Validation of the Estimation of Low-density LipoproteinCholesterol by the Modified Friedewald Equation in EthnicChinese Adults Living in Taiwan. Internal Medicine, 54: 2291-2297, 2015
[3]. Fukui K, et al. Effect of Cholesterol Reduction on Receptor Signaling in Neurons. J Biol Chem. 2015 Sep 14.
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5863 mL | 12.9316 mL | 25.8632 mL |
| 5 mM | 0.5173 mL | 2.5863 mL | 5.1726 mL |
| 10 mM | 0.2586 mL | 1.2932 mL | 2.5863 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。