Chondroitin Sulfate A (sodium salt)
(Synonyms: Chondroitin-4-sulfate,CSA) 目录号 : GC91489Chondroitin sulfate A (CSA) is a glycosaminoglycan.
Cas No.: 9082-07-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
Chondroitin sulfate A (CSA) is a glycosaminoglycan.[1] It can be biosynthetically polymerized in a variety of species and serve as structural components in cartilage when tethered to extracellular proteins. CSA (0.5 or 1 mg/ml) reduces LPS-induced decreases in viability, as well as LPS-induced increases in Tnf-α, Il-1β, Il-6, Ifn-γ, inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-1 (Mmp-1) levels, nitric oxide (NO) production, and NF-κB activity in primary mouse chondrocytes.[2] Extracellular CSA is required for P. falciparum-infected erythrocytes to adhere to the endothelium in the brain and placenta and avoid immune detection.[3] In vivo, CSA (30, 60, or 120 mg/kg per day) reduces arthritis incidence and joint swelling, decreases 8-isoprostane levels in articular cartilage cells, and increases IκBα levels in cartilage cell cytoplasm in a mouse model of collagen-induced arthritis.[4]
References:
[1].Kwok, J.C.F., Warren, P., and Fawcett, J.W.Chondroitin sulfate: A key molecule in the brain matrixInt. J. Biochem. Cell Biol.44(4)582-586(2012).
[2].Campo, G.M., Avenoso, A., Campo, S., et al.Glycosaminoglycans modulate inflammation and apoptosis in LPS-treated chondrocytesJ. Cell. Biochem.106(1)83-92(2009).
[3].Reeder, J.C., Cowman, A.F., Davern, K.M., et al.The adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A is mediated by P. falciparum erythrocyte membrane protein 1Proc. Natl. Acad. Sci. U.S.A.96(9)5198-5120(1999).
[4].Campo, G.M., Avenoso, A., Campo, S., et al.Chondroitin-4-sulphate inhibits NF-kB translocation and caspase activation in collagen-induced arthritis in miceOsteoarthritis Cartilage16(12)1474-1483().
Cas No. | 9082-07-9 | SDF | Download SDF |
别名 | Chondroitin-4-sulfate,CSA | ||
分子式 | C14H22NO15S•Na | 分子量 | 499.4 |
溶解度 | PBS (pH 7.2): 2 mg/ml | 储存条件 | -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.0024 mL | 10.012 mL | 20.024 mL |
5 mM | 0.4005 mL | 2.0024 mL | 4.0048 mL |
10 mM | 0.2002 mL | 1.0012 mL | 2.0024 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。