Cirsiliol
(Synonyms: 条叶蓟素) 目录号 : GC31235
A flavonoid with diverse biological activities
Cas No.:34334-69-5
Sample solution is provided at 25 µL, 10mM.
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Cirsiliol is a flavonoid that has been found in S. indicum and has diverse biological activities.1 It inhibits 5-lipoxygenase (5-LO) and 12-LO (IC50s = 0.1 and 1 ?M, respectively).1 Cirsiliol inhibits the release of slow-reacting substance of anaphylaxis (SRS-A) in passively sensitized isolated guinea pig lung (IC50 = 0.4 ?M). It induces relaxation of precontracted isolated rat uterus, urinary bladder, proximal aorta, and trachea in a concentration-dependent manner.2 Cirsiliol inhibits colony formation and migration of B16/F10 murine melanoma cells.3 In vivo, cirsiliol (200 ?g/kg) enhances radiation-induced inhibition of tumor growth in an H1299 non-small cell lung cancer (NSCLC) mouse xenograft model.4
1.Yoshimoto, T., Furukawa, M., Yamamoto, S., et al.Flavonoids: Potent inhibitors of arachidonate 5-lipoxygenaseBiochem. Biophys. Res. Commun.116(2)612-618(1983) 2.Mustafa, E.H., Zarga, M.A., Sabri, S., et al.Effects of cirsiliol, a flavone isolated from Achillea fragrantissima, on rat isolated smooth muscleInt. J. Pharm.33(3)204-209(1995) 3.Prasad, P., Vasas, A., Hohmann, J., et al.Cirsiliol suppressed epithelial to mesenchymal transition in B16F10 malignant melanoma cells through alteration of the PI3K/Akt/NF-κB signaling pathwayInt. J. Mol. Sci.20(3)608(2019) 4.Kang, J., Kim, E., Kim, W., et al.Rhamnetin and cirsiliol induce radiosensitization and inhibition of epithelial-mesenchymal transition (EMT) by miR-34a-mediated suppression of Notch-1 expression in non-small cell lung cancer cell linesJ. Biol. Chem.288(38)27343-27357(2013)
Cell experiment: | NSCLC, NCI-H1299, NCI-H460, WI-26 VA4 and MRC-5 cell lines are exposed to a single dose of γ-rays. Cells are then treated with rhamnetin and cirsiliol (5, 10, 15, 20, 25 μM) dissolved in DMSO for 4 h[2]. |
Animal experiment: | Mice[1]BALB/c athymic nude mice are injected with 2×106 NCI-H1299 cells. When the tumor has acquired a minimal volume of 200 mm3, DMSO or Cirsiliol (200 μg/kg body weight) is administered intraperitoneally every day for 25 days. The animals are also irradiated with 10 Gy once a week for 3 weeks. On day 25, the tumors are excised and subjected to further analyses[1]. |
References: [1]. Mustafa EH, et al. Effects of cirsiliol, a flavone isolated from Achillea fragrantissima, on rat isolated ileum. Gen Pharmacol. 1992 May;23(3):555-60. | |
| Cas No. | 34334-69-5 | SDF | |
| 别名 | 条叶蓟素 | ||
| Canonical SMILES | O=C1C=C(C2=CC=C(O)C(O)=C2)OC3=CC(OC)=C(OC)C(O)=C13 | ||
| 分子式 | C17H14O7 | 分子量 | 330.29 |
| 溶解度 | DMSO : ≥ 25 mg/mL (75.69 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0276 mL | 15.1382 mL | 30.2764 mL |
| 5 mM | 0.6055 mL | 3.0276 mL | 6.0553 mL |
| 10 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet