Citrullinated Vimentin (G146R) (R144 + R146) (139-159)-biotin Peptide
(Synonyms: Biotin-GQGKS(Cit)L(Cit)DLYEEEMRELRRQ, Biotin-GQGKSXLXDLYEEEMRELRRQ (X=Citrulline), Citrullinated VIM (G146R) (R144 + R146)-biotin) 目录号 : GC52367A biotinylated and citrullinated mutant vimentin peptide
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Citrullinated vimentin (G146R) (R144 + R146) (139-159)-biotin peptide is a biotinylated version of a di-citrullinated form of a mutant isoform of the cytoskeleton intermediate filament protein vimentin, vimentinG146R, which has been found in the synovial fluid of patients with rheumatoid arthritis.1 Vimentin is subject to citrullination by peptidyl arginine deiminase 2 (PAD2) under high calcium concentrations, which can occur during macrophage apoptosis.2,3 Serum levels of immune complexes targeting citrullinated vimentin are increased in patients with rheumatoid arthritis and positive for anti-citrullinated protein antibodies (ACPAs).4 Serum and plasma levels of citrullinated vimentin are also increased in patients with lung cancer or idiopathic pulmonary fibrosis, respectively.5,6 's Citrullinated Vimentin (G146R) (R144 + R146) (139-159)-biotin Peptide is intended for use in the detection of autoantibodies against vimentinG146R citrullinated at R144 and R146.
1.Bang, H., Egerer, K., Gauliard, A., et al.Mutation and citrullination modifies vimentin to a novel autoantigen for rheumatoid arthritisArthritis Rheum.56(8)2503-2511(2007) 2.Li, F.J., Surolia, R., Li, H., et al.Citrullinated vimentin mediates development and progression of lung fibrosisSci. Transl. Med.13(585)eaba2927(2021) 3.Asaga, H., Yamada, M., and Senshu, T.Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse peritoneal macrophagesBiochem. Biophys. Res. Commun.243(3)641-646(1998) 4.Van Steendam, K., Tilleman, K., De Ceuleneer, M., et al.Citrullinated vimentin as an important antigen in immune complexes from synovial fluid of rheumatoid arthritis patients with antibodies against citrullinated proteinsArthritis Res. Ther.12(4)R132(2010) 5.Willumsen, N., Bager, C.L., Leeming, D.J., et al.Serum biomarkers reflecting specific tumor tissue remodeling processes are valuable diagnostic tools for lung cancerCan. Med.3(5)1136-1145(2014) 6.Li, F.J., Surolia, R., Li, H., et al.Autoimmunity to vimentin is associated with outcomes of patients with idiopathic pulmonary fibrosisJ. Immunol.199(5)1596-1605(2017)
| Cas No. | SDF | Download SDF | |
| 别名 | Biotin-GQGKS(Cit)L(Cit)DLYEEEMRELRRQ, Biotin-GQGKSXLXDLYEEEMRELRRQ (X=Citrulline), Citrullinated VIM (G146R) (R144 + R146)-biotin | ||
| 分子式 | 分子量 | ||
| 溶解度 | Soluble in Water | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。