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Citrulline-specific Probe-Rhodamine Sale

(Synonyms: Rhodamine Phenylglyoxal, RhPG) 目录号 : GC43275

A fluorophore that detects protein citrullination

Citrulline-specific Probe-Rhodamine Chemical Structure

Cas No.:2309313-01-5

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250μg
¥2,381.00
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500μg
¥4,283.00
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1mg
¥7,623.00
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Sample solution is provided at 25 µL, 10mM.

Description

Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form citrulline, which plays a large role in regulating gene expression. Abnormally high PAD activity has been observed in a host of human diseases. Citrulline-specific probe-rhodamine is a highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline. This chemical probe (comprised of a single isomer) is capable of reacting with any citrulline-containing protein and can be analyzed with fluorescent imaging (excitation 532 nm; emission 580 nm). When added at 100 µM for 30 minutes at acidic pH, this probe has a reported limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4.

化学性质

Cas No. 2309313-01-5 SDF
别名 Rhodamine Phenylglyoxal, RhPG
Canonical SMILES CN(C)C1=CC2=C(C=C1)C3(C(C=CC(C(NCC4=CN(C(C(NC5=CC(C(C([H])=O)=O)=CC=C5)=O)C)N=N4)=O)=C6)=C6C(O3)=O)C7=CC=C(N(C)C)C=C7O2.O
分子式 C39H35N7O7•H2O 分子量 731.8
溶解度 Acetonitrile:Water (2:1): 1 mg/ml,DMF: 1 mg/ml 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

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1 mg 5 mg 10 mg
1 mM 1.3665 mL 6.8325 mL 13.6649 mL
5 mM 0.2733 mL 1.3665 mL 2.733 mL
10 mM 0.1366 mL 0.6832 mL 1.3665 mL
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