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CKMT1 Human

目录号 : GP22464

Creatine Kinase Muscle Type-1 Human Recombinant

CKMT1 Human Chemical Structure

规格 价格 库存 购买数量
10μg
¥840.00
5-10工作日
50μg
¥2,030.00
5-10工作日
1mg
¥13,230.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

Description

CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined bya) Analysis by RP-HPLCb) Analysis by SDS-PAGE Source Pichia Pastoris.
Phycical Appearance Sterile Filtered colourless liquid formulation. Shipping Condition Shipped with Ice Packs.
Synonyms Creatine kinase M-type; EC 2.7.3.2; Creatine kinase M chain; M-CK; CKM; CKMM; CKMMITI.
Stability CKMT1 although stable at 15°C for 7 days, should be stored below -18°C . Please prevent freeze-thaw cycles.
Biological Activity The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 ?mole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.
Formulation CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.

Introduction

Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Biological Activity

The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 ?mole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.

Stability

CKMT1 although stable at 15°C for 7 days, should be stored below -18°C . Please prevent freeze-thaw cycles.

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