Clathrin-IN-1
目录号 : GC70227Clathrin-IN-1是一种选择性网格蛋白介导的内吞作用(CME)抑制剂。
Cas No.:1332879-52-3
Sample solution is provided at 25 µL, 10mM.
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Preincubation of HeLa cells with Clathrin-IN-1 (Pitstops 2) leads to a dose-dependent inhibition of Tf uptake with an IC50 value (12-5 μM). Application of 30 μM Clathrin-IN-1 completely blocked Tf endocytosis. Clathrin-IN-1-induced block of Tf endocytosis in HeLa cells was completely reversed within 1-3 hr of drug washout. In U2OS cells, the IC50 for Tf uptake is 9.7 μM. Pitstop 2 also causes a potent inhibition of EGF uptake[1].
Clathrin-IN-1 (Pitstops 2) potently and specifically reduced HIV-1 infectivity by >90% in HeLa cells[1].
Pitstop-induced inhibition of clathrin TD function acutely interferes with receptor-mediated endocytosis, entry of HIV, and synaptic vesicle recycling. Endocytosis inhibition is caused by a dramatic increase in the lifetimes of clathrin coat components, including FCHo, clathrin, and dynamin, suggesting that the clathrin TD regulates coated pit dynamics[1].
References:
[1]. Lisa von Kleist, et al. Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition. Cell. 2011 Aug 5;146(3):471-84.
Cas No. | 1332879-52-3 | SDF | |
分子式 | C20H13BrN2O3S2 | 分子量 | 473.36 |
溶解度 | 储存条件 | ||
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1 mg | 5 mg | 10 mg | |
1 mM | 2.1126 mL | 10.5628 mL | 21.1256 mL |
5 mM | 0.4225 mL | 2.1126 mL | 4.2251 mL |
10 mM | 0.2113 mL | 1.0563 mL | 2.1126 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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