Coelenterazine 400a (hydrochloride)
目录号 : GC43291A bisdeoxy derivative of coelenterazine
Cas No.:2320429-05-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).[1],[2] It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h , which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.3 Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.[2],[3] BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.[1],[2],[4]
Reference:
[1]. Bertrand, L., Parent, S., Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS). J. Recept. Signal Transduct. Res. 22(1-4), 533-541 (2002).
[2]. Galés, C., Rebois, R.V., Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells. Nat. Methods 2(3), 177-184 (2005).
[3]. Pfleger, K.D., and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3(3), 165-174 (2006).
[4]. Huang, Q., Acha, V., Yow, R., et al. Bioluminescence measurements in mice using a skin window. J. Biomed. Opt. 12(5):054012, (2007).
| Cas No. | 2320429-05-6 | SDF | |
| 化学名 | 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one, monohydrochloride | ||
| Canonical SMILES | O=C1C(CC2=CC=CC=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31.Cl | ||
| 分子式 | C26H21N3O•HCl | 分子量 | 427.9 |
| 溶解度 | 0.5mg/mL in DMSO, slightly soluble in ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.337 mL | 11.685 mL | 23.3699 mL |
| 5 mM | 0.4674 mL | 2.337 mL | 4.674 mL |
| 10 mM | 0.2337 mL | 1.1685 mL | 2.337 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。