Complete Freund's adjuvant (CFA)
目录号 : GC68893Complete Freund's adjuvant (CFA)是一种参与抗原乳化的免疫佐剂，用于增强动物对抗原的免疫反应，因发现者Jules T. Freund而获名。
Sample solution is provided at 25 µL, 10mM.
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Complete Freund's adjuvant (CFA) is an immune adjuvant that participates in the emulsification of antigens and is used to enhance the immune response of animals to antigens. It is named after its discoverer, Jules T. Freund[1]. Complete Freund's adjuvant (CFA) is not only a Th1 polarizing adjuvant, but also a potent inducer of IL-17 immunity[2]. Complete Freund's adjuvant (CFA) contains heat-killed, inactivated Mycobacterium tuberculosis and is composed of a paraffin oil-in-water emulsion[3]. Complete Freund's adjuvant (CFA) stimulates a strong and lasting immune response and can be used to induce a persistent inflammatory pain model in mice, an experimental autoimmune myocarditis (EAM) model, etc.[4]. Incomplete Freund's adjuvant (IFA) is another type of Freund's adjuvant that stimulates a weaker immune response[5].
In vivo, Complete Freund's adjuvant (CFA) (0.1mg) was administered subcutaneously to SD rats to induce rheumatoid arthritis (RA)[6]. Complete Freund's adjuvant (CFA) (0.1mg) was administered subcutaneously to rats to induce skin inflammation[7].
References:
[1] Billiau A, Matthys P. Modes of action of Freund's adjuvants in experimental models of autoimmune diseases[J]. Journal of leukocyte biology, 2001, 70(6): 849-860.
[2] Tigno-Aranjuez J T, Lehmann P V, Tary-Lehmann M. Dissociated induction of cytotoxicity and DTH by CFA and CpG[J]. Journal of Immunotherapy, 2009, 32(4): 389-398.
[3] Skupnevskiy S V, Trukhina G M, Pukhaeva E G, et al. Therapeutic Effects of Citric and Succinic Acids in Rats Exposed to Inactivated M. tuberculosis[J]. Public Health and Life Environment–PH&LE, 2021, 29(8): 69-75.
[4] Fontes J A, Barin J G, Talor M V, et al. Complete Freund's adjuvant induces experimental autoimmune myocarditis by enhancing IL‐6 production during initiation of the immune response[J]. Immunity, inflammation and disease, 2017, 5(2): 163-176.
[5] Shibaki A, Katz S I. Induction of skewed Th1/Th2 T‐cell differentiation via subcutaneous immunization with Freund's adjuvant[J]. Experimental dermatology, 2002, 11(2): 126-134.
[6] Weng W, Wang F, He X, et al. Protective effect of corynoline on the CFA induced rheumatoid arthritis via attenuation of oxidative and inflammatory mediators[J]. Molecular and Cellular Biochemistry, 2021, 476: 831-839.
[7] Fehrenbacher J C, Vasko M R, Duarte D B. Models of inflammation: carrageenan‐or complete freund's adjuvant (CFA)–induced edema and hypersensitivity in the rat[J]. Current protocols in pharmacology, 2012, 56(1): 5.4. 1-5.4. 4.
Complete Freund's adjuvant (CFA)是一种参与抗原乳化的免疫佐剂，用于增强动物对抗原的免疫反应，因发现者Jules T. Freund而获名[1]。Complete Freund's adjuvant (CFA)不仅是Th1极化佐剂，也是IL-17免疫的强效诱导剂[2]。Complete Freund's adjuvant (CFA)含有热灭活的非活性化结核杆菌，并由石蜡油包水乳剂组成[3]。Complete Freund's adjuvant (CFA)刺激强烈且持久的免疫反应，可用于诱导小鼠的持续性炎症疼痛模型、实验性自身免疫性心肌炎（EAM）模型等[4]。Incomplete Freund's adjuvant (IFA)是Freund's Adjuvant的另一种类型，刺激的免疫反应较弱[5]。
在体内，Complete Freund's adjuvant (CFA)（0.1mg）通过皮下注射处理SD大鼠，诱导了类风湿性关节炎（RA）[6]。Complete Freund's adjuvant (CFA)（0.1mg）通过皮下注射处理大鼠，诱导了皮肤炎症[7]。
| Animal experiment [1]: | |
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Animal models |
Sprague-Dawley rats |
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Preparation Method |
The rats were injected subcutaneously with 0.1mL Complete Freund's adjuvant (CFA) (1mg/mL) into the foot pad of the left hind paw. A booster subcutaneous injection of 0.1mL was given into the tail on the same day and on the following day. |
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Dosage form |
0.1mg, once a day for two days; s.c. |
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Applications |
Complete Freund's adjuvant (CFA) induced rheumatoid arthritis (RA) in experimental rats. |
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References: |
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| Cas No. | SDF | Download SDF | |
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | Store at 2-8°C,protect from light | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
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