Concanavalin A
(Synonyms: 刀豆蛋白) 目录号 : GC18572
Concanavalin A属于concanamycin，这是一种从异色链霉菌中分离出来的大环内酯类抗生素，是液泡质子- atp酶(v-[H[+]] atp酶)的高活性和选择性抑制剂。
Cas No.:11028-71-0
Sample solution is provided at 25 µL, 10mM.
Concanavalin A belongs to the concanamycins, a family of macrolide antibiotics isolated from Streptomyces diastatochromogenes that are highly active and selective inhibitors of the vacuolar proton-ATPase (v-[H[+]]ATPase). Among them, Concanavalin A is the most selective and potent inhibitor of the V-[H[+]]ATPase.[1]
n vitro study indicated that Concanavalin A was active at 5 nM and completely blocked influenza virus infection at 10 nM in MDCK cells. Results showed that concanamycin A blocked viral replication by inhibiting the v-[H[+]]ATPase, thus preventing acidification of endosomes and release of virions into the cytoplasm. An early event in virus infection is the target of concanamytin A. In addition, the inhibition of the v-[H[+]]ATPase by concanamycin A prevents endosomal acidification, inhibiting virus release from endosomes.[1]
References:
[1].Rosario G. et al. Concanavalin A blocks influenza virus entry into cells under acidic conditions. FEBS Letters 1994 June; 349; 327-330.
| Cell experiment [1]: | |
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Cell lines |
CD8+ CTL |
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Preparation Method |
Cells were cultured in the RPMI 1640 medium supplemented with 10% (v/v) FCS and 5% (v/v) culture supernatant of rat spleen cells. |
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Reaction Conditions |
Cells were stimulated with 5 μg/ml of concanavalin A for 24 h. |
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Applications |
Concanavalin A treatment could preferentially decrease the cell viability of CD8+ population prepared from the immunized mice. A certain group(s) of CD8+ population in the immunized mice might be highly susceptible to concanavalin A. The activation through T cell receptors, especially PKC activation, increases the sensitivity of CD8++ CTL to concanavalin A. |
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References: [1]. Kenichi T. et al. Concanamycin A, a vacuolar type H+-ATPase inhibitor, induces cell death in activated CD8+ CTL. Cytotechnology 1997 July; 25: 127–135. |
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| Cas No. | 11028-71-0 | SDF | |
| 别名 | 刀豆蛋白 | ||
| 分子式 | 分子量 | ||
| 溶解度 | Soluble in water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet