CORM-3
(Synonyms: (OC-6-44)-三羰基氯(甘氨酸基)钌) 目录号 : GC18071
CORM-3是一种水溶性CO释放分子，通过与磷酸盐载体的相互作用解除线粒体呼吸作用。
Cas No.:475473-26-8
Sample solution is provided at 25 µL, 10mM.
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CORM-3 is a water soluble CO-releasing molecule, uncouples mitochondrial respiration via interaction with the phosphate carrier. It can reduce the nuclear heterotopic of NF-κB p65, reduce the production of ROS, and increase the levels of glutathione and superoxide dismutase in cells. Besides, CORM-3 reduces the activation of NLRP3 inflammasome [1-3].
CORM-3(0-400 μM; 2 hours) inhibited the effect of H2O2 on the viability of BMSCs in a dose-dependent manner. CORM-3 could reduce reactive oxygen species (ROS) accumulation and prevent mitochondrial dysfunction thereby restoring the osteogenic potential of the BMSCs disrupted by hydrogen peroxide (H2O2) exposure[4]. CORM-3(20 μM ;30 min) induces DNA damage through Ru(II) binding to DNA[5].
CORM-3(4 mg/kg; i.v) mitigated HSR-induced intestinal damages[6]. CORM-3 could effectively improve the inflammatory response induced by activation of the microglia (MG), reduce neuronal apoptosis, promote neural regeneration, and improve the learning and memory performance of mice after radiation[7].
References:
[1]. Long R, Salouage I, Berdeaux A, et,al. CORM-3, a water soluble CO-releasing molecule, uncouples mitochondrial respiration via interaction with the phosphate carrier. Biochim Biophys Acta. 2014 Jan;1837(1):201-9. doi: 10.1016/j.bbabio.2013.10.002. Epub 2013 Oct 23. PMID: 24161358.
[2]. Huang Y, Ma T, et,al. Carbon monoxide (CO) inhibits hydrogen peroxide (H2O2)-induced oxidative stress and the activation of NF-κB signaling in lens epithelial cells. Exp Eye Res. 2018 Jan;166:29-39. doi: 10.1016/j.exer.2017.08.016. Epub 2017 Oct 16. PMID: 29051011.
[3]. Lee DW, Shin HY, et,al. Carbon monoxide regulates glycolysis-dependent NLRP3 inflammasome activation in macrophages. Biochem Biophys Res Commun. 2017 Nov 18;493(2):957-963. doi: 10.1016/j.bbrc.2017.09.111. Epub 2017 Sep 21. PMID: 28942141.
[4]. Jin C, Lin BH, et,al. CORM-3 Attenuates Oxidative Stress-Induced Bone Loss via the Nrf2/HO-1 Pathway. Oxid Med Cell Longev. 2022 Aug 17;2022:5098358. doi: 10.1155/2022/5098358. PMID: 36035220; PMCID: PMC9402314.
[5]. Lyon RF, Southam HM, et,al. CORM-3 induces DNA damage through Ru(II) binding to DNA. Biochem J. 2022 Jul 15;479(13):1429-1439. doi: 10.1042/BCJ20220254. PMID: 35726678; PMCID: PMC9342897.
[6]. Zhang LM, Xin Y, et,al. CORM-3 alleviates the intestinal injury in a rodent model of hemorrhage shock and resuscitation: roles of GFAP-positive glia. J Mol Histol. 2023 Aug;54(4):271-282. doi: 10.1007/s10735-023-10133-w. Epub 2023 Jun 19. PMID: 37335421.
[7]. Lu K, Wu WJ, et,al. CORM-3 Regulates Microglia Activity, Prevents Neuronal Injury, and Improves Memory Function During Radiation-induced Brain Injury. Curr Neurovasc Res. 2020;17(4):464-470. doi: 10.2174/1567202617999200730213259. PMID: 32748746.
CORM-3是一种水溶性CO释放分子，通过与磷酸盐载体的相互作用解除线粒体呼吸作用。它可以减少NF-κB p65的核异位，减少ROS的产生，增加细胞中谷胱甘肽和超氧化物歧化酶的水平。此外，CORM-3可降低NLRP3炎性体的活化[1-3]。
CORM-3(0-400 μM; 2 hours)抑制H2O2对骨髓间充质干细胞活力的影响呈剂量依赖性。CORM-3可以减少活性氧(ROS)的积累，防止线粒体功能障碍，从而恢复被过氧化氢破坏的骨髓间充质干细胞的成骨潜能[4]。CORM-3(20 μM ;30 min)通过Ru(II)与DNA的结合诱导DNA损伤[5]。
CORM-3(4 mg/kg; i.v)减轻HSR引起的肠道损伤[6]。CORM-3能有效改善小神经胶质活化引起的炎症反应，减少神经元凋亡，促进神经再生，提高辐射后小鼠的学习记忆能力[7]。
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Cell experiment [1]: |
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Cell lines |
BMSCs (Sprague-Dawley rats) |
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Preparation method |
Cells were inoculated in 96-well plates at a density of and treated for 2 hours with varying CORM-3 concentrations (0, 12.5, 25, 50, 100, 200, and 400 μM) with or without H2O2 treatment. |
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Reaction Conditions |
0, 12.5, 25, 50, 100, 200, and 400 μM; 2 hours |
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Applications |
CORM-3 inhibited the effect of H2O2 on the viability of BMSCs in a dose-dependent manner. |
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Animal experiment [2]: |
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Animal models |
Male SD rats (Hemorrhagic shock and resuscitation (HSR)model) |
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Preparation method |
HSR rat were intravenously administered with CORM-3. At 24 h and 7 d after HSR modeling, the pathological changes in intestinal tissues were evaluated. |
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Dosage form |
4 mg/kg; i.v |
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Applications |
CORM-3 mitigated HSR-induced intestinal damages. |
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References: [1].Jin C, Lin BH, et,al. CORM-3 Attenuates Oxidative Stress-Induced Bone Loss via the Nrf2/HO-1 Pathway. Oxid Med Cell Longev. 2022 Aug 17;2022:5098358. doi: 10.1155/2022/5098358. PMID: 36035220; PMCID: PMC9402314. [2]. Zhang LM, Xin Y, et,al. CORM-3 alleviates the intestinal injury in a rodent model of hemorrhage shock and resuscitation: roles of GFAP-positive glia. J Mol Histol. 2023 Aug;54(4):271-282. doi: 10.1007/s10735-023-10133-w. Epub 2023 Jun 19. PMID: 37335421. |
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| Cas No. | 475473-26-8 | SDF | |
| 别名 | (OC-6-44)-三羰基氯(甘氨酸基)钌 | ||
| Canonical SMILES | O=C1[O-][Ru+2]([Cl-])([C]=O)([C]=O)([C]=O)[NH2]C1 | ||
| 分子式 | C5H4ClNO5Ru | 分子量 | 294.61 |
| 溶解度 | DMSO : 100 mg/mL; Water : 50 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3943 mL | 16.9716 mL | 33.9432 mL |
| 5 mM | 0.6789 mL | 3.3943 mL | 6.7886 mL |
| 10 mM | 0.3394 mL | 1.6972 mL | 3.3943 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet