Cosyntropin (acetate)
(Synonyms: 醋酸替可克肽,Tetracosactrin acetate) 目录号 : GC18804
Cosyntropin (acetate)是一种合成肽，可刺激肾上腺释放皮质类固醇，如皮质醇。Cosyntropin (acetate)目前用于研究溃疡性结肠炎和克罗恩病、幼年/成人类风湿性关节炎和骨关节病。
Cas No.:60189-34-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Adrenal cells |
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Preparation Method |
Adrenal cells were treated with 10nM Cosyntropin (acetate) for 48 hours. |
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Reaction Conditions |
10nM; 48h |
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Applications |
After Cosyntropin (acetate)-stimulated conditions, PregS and Preg demonstrated abrupt acute responses (21-and 12-fold increase at 3 hours compared with baseline, respectively) and peaked after 24 hours. In contrast, DHEAS and DHEA responded only modestly to Cosyntropin (acetate) stimulation (1.8- and 2.8-fold increase at 3 hours compared with baseline, respectively). By 48 hours after Cosyntropin (acetate) stimulation, a modest decline in PregS levels and a steep drop in Preg content, whereas the DHEAS level continued to rise slightly. |
| Animal experiment [2]: | |
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Animal models |
Glucocorticoid-induced osteonecrosis model |
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Preparation Method |
We treated female rabbits averaging 4.48 kg with depot methylprednisolone acetate alone or Cosyntropin (acetate) 0.2μg/kg, daily, as an sc. injection. They were also administered 10 mg/kg tetracycline 5 days apart before sacrifice. |
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Dosage form |
0.2μg/kg/day; SC; 24d |
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Applications |
Cosyntropin (acetate) stimulates vascular endothelial growth factor production in vivo and induces vascular endothelial growth factor secretion from osteoblasts via the pro-adrenocorticotropic hormone receptor, MC2R, which supports osteoblast maturation and survival in vitro. |
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References: |
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Cosyntropin (acetate) is a synthetic peptide stimulating the release of corticosteroids such as cortisol from the adrenal gland. Cosyntropin (acetate) is currently used for the research of ulcerative colitis and Crohn's disease, juvenile/adult rheumatoid arthritis and osteoarthrosis[1].
Under the stimulation of Cosyntropin (acetate) (10nM; 48h), PregS and Preg in adrenal cells showed an acute response (increased 21-fold and 12-fold respectively over the baseline within 3h) and reached a peak after 24h. In contrast, DHEAS and DHEA did not respond much to Cosyntropin (acetate) stimulation (increased 1.8-fold and 2.8-fold respectively over the baseline after 3h). After 48h of Cosyntropin (acetate) stimulation, PregS levels decreased slightly, Preg content decreased sharply, and DHEAS levels continued to increase slightly [2].
Cosyntropin (acetate) (0.2μg/kg/day; SC; 24d) stimulates vascular endothelial growth factor production in vivo and induces vascular endothelial growth factor secretion from osteoblasts via the pro-adrenocorticotropic hormone receptor, MC2R, which supports osteoblast maturation and survival in vitro[3]. Reduced microglia/macrophage accumulation and neutrophil infiltration in conjunction with moderate improvements in spatial learning in Cosyntropin (acetate) treated TBI mice[4].
References:
[1]Iuliano F, Iuliano E, Rossi M. Adding Slow Release Tetracosactide Acetate to Eltrombopag Improves Haematological Response in ITP Patients with Platelet-Count Fluctuation[J]. Blood, 2018, 132: 2449.
[2]. Rege J, Nanba A T, Auchus R J, et al. Adrenocorticotropin acutely regulates pregnenolone sulfate production by the human adrenal in vivo and in vitro[J]. The Journal of Clinical Endocrinology & Metabolism, 2018, 103(1): 320-327.
[3].Zaidi M, Sun L, Robinson L J, et al. ACTH protects against glucocorticoid-induced osteonecrosis of bone[J]. Proceedings of the National Academy of Sciences, 2010, 107(19): 8782-8787.
[4]. Siebold L, Krueger A C, Abdala J A, et al. Cosyntropin attenuates neuroinflammation in a mouse model of traumatic brain injury[J]. Frontiers in Molecular Neuroscience, 2020, 13: 109.
Cosyntropin (acetate)是一种合成肽，可刺激肾上腺释放皮质类固醇，如皮质醇。Cosyntropin (acetate)目前用于研究溃疡性结肠炎和克罗恩病、幼年/成人类风湿性关节炎和骨关节病[1]。
在Cosyntropin (acetate)（10nM；48h）刺激条件下，肾上腺细胞中PregS和Preg表现出急性反应（3h内分别比基线增加21倍和12倍），并在 24h后达到峰值。相比之下，DHEAS和DHEA对Cosyntropin (acetate)的刺激反应不大（3h后分别比基线增加1.8倍和2.8倍）。Cosyntropin (acetate)刺激48h后，PregS水平略有下降，Preg含量急剧下降，而DHEAS水平继续小幅上升[2]。
在糖皮质激素诱导的骨坏死模型中，Cosyntropin (acetate)（0.2μg/kg/day）可刺激体内血管内皮生长因子的产生，并通过促肾上腺皮质激素受体MC2R诱导成骨细胞分泌血管内皮生长因子，从而支持体外成骨细胞的成熟和存活[3]。经Cosyntropin (acetate)治疗的创伤性脑损伤小鼠的小胶质细胞/巨噬细胞聚集和中性粒细胞浸润减少，空间学习能力得到适度改善[4]。
| Cas No. | 60189-34-6 | SDF | |
| 别名 | 醋酸替可克肽,Tetracosactrin acetate | ||
| 化学名 | α1-24-corticotropin, acetate | ||
| Canonical SMILES | N/A | ||
| 分子式 | C136H210N40O31S.XC2H4O2 | 分子量 | 2993.5 |
| 溶解度 | PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3341 mL | 1.6703 mL | 3.3406 mL |
| 5 mM | 0.0668 mL | 0.3341 mL | 0.6681 mL |
| 10 mM | 0.0334 mL | 0.167 mL | 0.3341 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
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