Cremophor EL
(Synonyms: 聚氧乙烯蓖麻油EL) 目录号 : GC30024
Cremophor EL (CrEL, Kolliphor EL), a polyethoxylated surfactant, is a formulation vehicle used for various poorly-water soluble drugs, including the anticancer agent paclitaxel (Taxol). Cremophor EL is used in severe anaphylactoid hypersensitivity reactions, hyperlipidaemia, abnormal lipoprotein patterns, aggregation of erythrocytes and peripheral neuropathy.
Cas No.:61791-12-6
Sample solution is provided at 25 µL, 10mM.
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Cremophor EL (CrEL, Kolliphor EL), a polyethoxylated surfactant, is a formulation vehicle used for various poorly-water soluble drugs, including the anticancer agent paclitaxel (Taxol). Cremophor EL is used in severe anaphylactoid hypersensitivity reactions, hyperlipidaemia, abnormal lipoprotein patterns, aggregation of erythrocytes and peripheral neuropathy.
Cremophor EL is a widely used excipient. In the first 10 h, Cremophor EL at 5 mg/mL increases the impedance, then decreases it, and after 20 h of treatment, epithelial cell death is detected. Cremophor EL in concentrations corresponding to clinical doses causes endothelial and epithelial toxicity. Both epithelial viability and monolayer integrity are seriously impaired at 10 to 50 mg/mL concentrations of Cremophor EL at 24-h treatment. Cremophor EL also enhances cell detachment.[1]
Cremophor EL reduces nociception in a dose-dependent manner. At 10.6 g/kg, Cremophor EL causes antinociception similar to that induced by dipyrone (300 mg/kg, by gavage) in the abdominal writhing test, and antinociception similar to that induced by morphine (20 mg/kg, by gavage) in the tail immersion test.
[1] Kiss L, et al. J Pharm Sci. 2013 Apr;102(4):1173-81. [3] Gelderblom H, et al. Eur J Cancer. 2001 Sep;37(13):1590-8.
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Cell experiment: |
Cell viability is determined by double fluorescent staining: bis-benzimide labels all cell nuclei, ethidium-homodimer-1 stains only dead cells. For morphological examinations, primary brain endothelial cells with tight barrier properties are used. Cells grown on glass coverslips are treated with Cremophor EL and RH40 for 1 and 24 h. Cremophor EL and RH40 are tested at 0.1 to 50 mg/mL concentrations. Triton X-100 detergent (10 mg/mL) is used in toxicity assays as a reference compound to cause cell death[1]. |
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References: [1]. Kiss L, et al. Kinetic analysis of the toxicity of pharmaceutical excipients Cremophor EL and RH40 on endothelial and epithelial cells. J Pharm Sci. 2013 Apr;102(4):1173-81. |
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| Cas No. | 61791-12-6 | SDF | |
| 别名 | 聚氧乙烯蓖麻油EL | ||
| Canonical SMILES | [Cremophor EL] | ||
| 分子式 | 分子量 | ||
| 溶解度 | Water : 25 mg/mL | 储存条件 | Store at RT, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet