Cu-ATSP

Cu-ATSP is a copper-containing bis(thiosemicarbazone) complex with ferroptosis inhibitory activity.¹ It traps peroxyl radicals in a cell-free assay when used at a concentration of 6 µM. Cu-ATSP inhibits ferroptosis induced by (1S,3R)-RSL3 in Pfa1 mouse embryonic fibroblasts (MEFs) and HT22 mouse hippocampal cells (EC₅₀s = 8.5 and 16 nM, respectively). It increases intracellular copper levels but, unlike the bis(thiosemicarbazone) complexes Cu-CQ2 and CuNC2, does not reduce amyloid-β (1-40) (Aβ40) levels in CHO cells overexpressing amyloid precursor protein (APP).²

1.Zilka, O., Poon, J.-F., and Pratt, D.A.Radical-trapping antioxidant activity of copper and nickel bis(thiosemicarbazone) complexes underlies their potency as inhibitors of ferroptotic cell deathJ. Am. Chem. Soc.143(45)19043-19057(2021) 2.Donnelly, P.S., Caragounis, A., Du, T., et al.Selective intracellular release of copper and zinc ions from bis(thiosemicarbazonato) complexes reduces levels of Alzheimer disease amyloid-β peptideJ. Biol. Chem.283(8)4568-4577(2008)