Cy3 carboxylic acid (non-sulfonated)
目录号 : GC17551
Non-reactive fluorophore, for control experiments, and for calibration
Sample solution is provided at 25 µL, 10mM.
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Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1].
Reference:
[1] Xue, M. ; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085–3093.
| Cas No. | SDF | ||
| 化学名 | 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride | ||
| Canonical SMILES | CC(C1=C(N/2C)C=CC=C1)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC=C4)=C4C3(C)C.[Cl-] | ||
| 分子式 | C30H37ClN2O2 | 分子量 | 493.08 |
| 溶解度 | ≥ 49.3mg/mL in DMSO, ≥ 99 mg/mL in EtOH | 储存条件 | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0281 mL | 10.1403 mL | 20.2807 mL |
| 5 mM | 0.4056 mL | 2.0281 mL | 4.0561 mL |
| 10 mM | 0.2028 mL | 1.014 mL | 2.0281 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet