Cy5.5 maleimide (non-sulfonated)
目录号 : GC10412
Labeling of cysteine residues, as well as other thiolated molecules
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cy5.5 maleimide is a thiol reactive dye which is capable of selective labeling of proteins which contain sulfhydryl groups. Cy5.5 maleimide has near infrared emission that makes it suitable for bioimaging applications. This reagent can replace Alexa Fluor 680 and DyLight 680. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cy5.5-NLP conjugate was synthesized by the NLP peptide and the Cy5.5-maleimide in 100 μL of anhydrous DMF, to which 10 μL of DIPEA was added. In the Her2/neu positive (SKBR-3 and BT474) or Her2/neunegative (MDA-MB-231) breast cancer cells, intracellular localization of Cy5.5-labeled peptide conjugates make the effects visible [1].
Reference:
[1]Cai, H. ; Singh, A.N.; Sun, X.; Peng, F. Synthesis and Characterization of Her2-NLP Peptide Conjugates Targeting Circulating Breast Cancer Cells: Cellular Uptake and Localization by Fluorescent Microscopic Imaging. Journal of Fluorescence, 2015, 25(1), 113–117.
| Cas No. | SDF | ||
| 化学名 | 6-[(2E)-1,1-dimethyl-2-[(2E,4E)-5-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)penta-2,4-dienylidene]benzo[e]indol-3-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide | ||
| Canonical SMILES | CC(/C1=C\C=C\C=C\C(C(C)2C)=[N+](C3=C2C4=CC=CC=C4C=C3)CCCCC/C([O-])=N/CCN5C(C=CC5=O)=O)(C6=C(N1C)C=CC7=CC=CC=C76)C.Cl | ||
| 分子式 | C46H49ClN4O3 | 分子量 | 741.36 |
| 溶解度 | ≥ 74.1mg/mL in DMSO | 储存条件 | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3489 mL | 6.7444 mL | 13.4887 mL |
| 5 mM | 0.2698 mL | 1.3489 mL | 2.6977 mL |
| 10 mM | 0.1349 mL | 0.6744 mL | 1.3489 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。