Cy5.5 NHS ester (non-sulfonated)
(Synonyms: CY5.5N-羟基琥珀酰亚胺酯) 目录号 : GC11653
Cy5.5-NHS 酯是一种近红外胺活性染料，用于标记肽、蛋白质和寡核苷酸的氨基。
Cas No.:1469277-96-0
Sample solution is provided at 25 µL, 10mM.
Cy5.5-NHS ester is a near-infrared amine reactive dye for labeling amino groups of peptides, proteins and oligonucleotides. Cy5.5-NHS ester is suitable for fluorescence analysis when the background fluorescence interferes. It can also be used for in vivo imaging experiments. Cy5.5-NHS ester can be dissolved in DMF and DMSO, the maximum excitation light and maximum emission light are around 675 nm and 693 nm, respectively.[1]
In vitro and in vivo experiment indicated that Cy5.5-NHS ester is a nice near-infrared fluorescent dye, which could be used for tumor targeting as well as examining the effect of D-glucose or L-glucose on 2-NBDG accumulation. Moreover, Cy5.5-NHS ester reacts swiftly that the tumor/muscle contrast could be clearly visualized from 30 min up to 24 h pi of 0.5 nmol Cy5.5-NHS. The tumor uptake of Cy5.5-NHS reached a maximum 30 minutes pi. [2]
References:
[1].Ju HR, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials. 2014. 35: 2302-2311.
[2].Zhen C. et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem. 2006 ; 17(3): 662–669.
Cy5.5-NHS 酯是一种近红外胺活性染料，用于标记肽、蛋白质和寡核苷酸的氨基。 Cy5.5-NHS ester 适用于背景荧光干扰时的荧光分析。它还可用于体内成像实验。 Cy5.5-NHS酯可溶于DMF和DMSO，最大激发光和最大发射光分别在675 nm和693 nm左右。[1]
体外和体内实验表明，Cy5.5-NHS酯是一种很好的近红外荧光染料，可用于肿瘤靶向以及检测D-葡萄糖或L-葡萄糖对2- NBDG积累。此外，Cy5.5-NHS 酯反应迅速，在 0.5 nmol Cy5.5-NHS 的感染后 30 分钟至 24 小时内，肿瘤/肌肉对比清晰可见。 Cy5.5-NHS 的肿瘤摄取在感染后 30 分钟达到最大值。 [2]
| Cell experiment [1]: | |
|
Cell lines |
U87MG |
|
Preparation Method |
U87MG cells (1 × 105) were cultured for 24 hours, then the cells were washed with PBS and then incubated at 37 °C in the presence of 100 nM Cy5.5-NHS ester for 10 minutes. After the incubation period, cells were washed three times with ice- cold PBS. |
|
Reaction Conditions |
The fluorescence signal of the cells was recorded using an Axiovert 200M fluorescence microscope equipped with a Cy5.5 filter set. |
|
Applications |
Cy5.5-NHS ester could be used for fluorescence microscopy imaging study with tumor cell lines such as U87MG, C6, A375M, B16F10 and MDAMB-435, as well as for examining the effect of D-glucose or L-glucose on 2-NBDG accumulation. |
| Animal experiment [1]: | |
|
Animal models |
Female athymic nude mice (nu/nu), 4–6 weeks |
|
Preparation Method |
Mice were subcutaneously injected with 5 × 106 U87MG glioblastoma cells suspended in 100 μL of PBS. When the tumors reached 0.4–0.6 cm in diameter, the tumor bearing mice were then injected via tail vein with Cy5.5-NHS ester and subjected to optical imaging at various time points postinjection (pi). |
|
Dosage form |
0.5 nmol |
|
Applications |
Cy5.5-NHS ester could be took advantage of tumor targeting, and the tumor/muscle contrast was clearly visible as early as 30 min postinjection. The highest U87MG tumor/muscle ratio of 3.34 ± 0.23 were achieved 24 hours post-injection for Cy5.5-NHS. |
|
References: [1]. Zhen C. et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem. 2006 ; 17(3): 662–669. |
|
| Cas No. | 1469277-96-0 | SDF | |
| 别名 | CY5.5N-羟基琥珀酰亚胺酯 | ||
| 化学名 | 3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium | ||
| Canonical SMILES | CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(ON4C(CCC4=O)=O)=O)=C1/C=C/C=C/C=C5C(C)(C)C6=C(C=CC=C7)C7=CC=C6N\5C | ||
| 分子式 | C44H46ClN3O4 | 分子量 | 716.31 |
| 溶解度 | ≥ 35.8mg/mL in DMSO | 储存条件 | Store at -20℃, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.396 mL | 6.9802 mL | 13.9604 mL |
| 5 mM | 0.2792 mL | 1.396 mL | 2.7921 mL |
| 10 mM | 0.1396 mL | 0.698 mL | 1.396 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
-
Related Biological Data
The preparation and characterizations of HMVs. (D) Co-localization analysis by confocal laser scanning microscopy (CLSM) observation of Cy5.5-labered K. pneumoniae OMVs (red fluorescence) and DiO-stained MHS MVs (green fluorescence) in the hybrid or the mixed MVs in a phagocytosis-based model.
Cy5.5 (GlpBio)-labeled HMVs were inoculated through the intratracheal instillation, and mice were imaged using the IVIS Lumina III imaging system on Day 1, 4 and 7 postinoculation.
Nano Today 54 (2024): 102135. IF: 17.4002 -
Related Biological Data
C) CLSM images (PHMG and CS were labeled with FITC and Cy5.5, respectively);
To more intuitively observe the chemical grafting PHMG and physical adsorption CS onto the surface of CG, PHMG and CS were first labeled with FITC and Cy5.5 (GlpBio), then chemically grafted and physically adsorbed onto the surface of OCG and CG, respectively, and finally observed by confocal laser scanning microscope.
Carbohydrate Polymers (2022): 119130. PMID: 35123752 IF: 9.384