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Cy5 amine (non-sulfonated) Sale

(Synonyms: Cyanine5 amine) 目录号 : GC14394

can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.

Cy5 amine (non-sulfonated) Chemical Structure

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1mg
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].

Reference:
[1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.

Chemical Properties

Cas No. SDF
别名 Cyanine5 amine
化学名 1-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium
Canonical SMILES CC1(C)C2=C(C=CC=C2)[N+](CCCCCC(NCCCCCC[NH3+])=O)=C1/C=C/C=C/C=C3C(C)(C)C4=CC=CC=C4N\3C
分子式 C38H54Cl2N4O 分子量 653.77
溶解度 ≥ 48mg/mL in DMSO, ≥ 8.84 mg/mL in EtOH 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.5296 mL 7.6479 mL 15.2959 mL
5 mM 0.3059 mL 1.5296 mL 3.0592 mL
10 mM 0.153 mL 0.7648 mL 1.5296 mL
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