Cy7.5 azide (non-sulfonated)
目录号 : GC10286
used for Click chemistry to label alkyne-modified oligonucleotides
Sample solution is provided at 25 µL, 10mM.
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Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. As generic Click Chemistry labeling protocol, azide is available as DMSO solution. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cy7.5 azide a sulfonated dye with absorption peaks at 773 and 808 nm, can be the optimal fluorescent probe that should have separate absorption spectra. The changes in the absorption and scattering properties of the tissue are negligible in the range of 750 to 850 nm [1].
Reference:
[1] Osnat Harbater,Israel Gannot. Fluorescent probes concentration estimation in vitro and ex vivo as a model for early detection of Alzheimer’s disease.Jornal of biomedical optics. 2014.19(12).
| Cas No. | SDF | ||
| 化学名 | N-(3-azidopropyl)-6-[(2E)-1,1-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]benzo[e]indol-3-yl]hexanamide | ||
| Canonical SMILES | CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC4=CC(=CC=C5C(C6=C(N5CCCCCC(=O)NCCCN=[N+]=[N-])C=CC7=CC=CC=C76)(C)C)CCC4)C | ||
| 分子式 | C48H55ClN6O | 分子量 | 767.44 |
| 溶解度 | soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water | 储存条件 | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.303 mL | 6.5152 mL | 13.0303 mL |
| 5 mM | 0.2606 mL | 1.303 mL | 2.6061 mL |
| 10 mM | 0.1303 mL | 0.6515 mL | 1.303 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet